摘要
选择超声波水提-醇沉法提取党参多糖,通过单因素实验和正交实验确定党参多糖的最佳提取工艺如下:党参干燥粉碎过60目筛,先后经石油醚、80%乙醇回流,挥干溶剂后,在提取温度为60℃、料液比为1∶12(g∶mL)、超声波功率为70%、提取时间为45min的条件下提取2次,党参多糖的提取率达到13.57%。对提取的党参多糖进行了抗氧化性研究,结果表明,当党参多糖浓度为0.4545μg·mL-1时,对羟自由基的清除率达到59.04%。
The ultrasonic-water extraction-ethanol precipitation method was selected to extract polysaccharides from Codonopsis pilosula. Through single factor experiment and orthogonal experiment, the optimum extraction process was obtained as follows：sieving the crushed Codonopsis pilosula using 60 mesh riddle, refluxing with petroleum ether, 80% ethanol, then drying to remove solvent, extracting twice under the conditions including extraction temperature of 60 ℃ ,solid-liquid ratio of 1 ： 12（g ： mL）,ultrasonic power of 70% ,extration time of 45 min, for which the extraction rate of polysaccharides from Codonopsis pilosula reached 13.57 %. Furthurmore,the antioxidant activity of the polysaccharides was studied. The result showed that the cleavage rate of hydroxyl radicals could reach 59.04% when the t）olvsaccharides concentration was 0. 4545 μg·mL-1.
出处
《化学与生物工程》
CAS
2012年第9期47-50,共4页
Chemistry & Bioengineering
基金
山西省自然科学基金资助项目(2008021015)
太原理工大学青年科技发展基金资助项目(900103-03020155)
关键词
党参多糖
提取
抗氧化性
polysaccharides of Codonopsis pilosula l extraction antioxidant activity
