摘要
目的:在肥胖与肥胖抵抗基础上,观察7周有氧运动对高脂膳食大鼠胰腺中神经肽Y(NPY)受体mRNA表达的影响。方法:80只SD大鼠高脂饲料喂养8周,根据体重筛选出肥胖和肥胖抵抗大鼠。分为6组:肥胖组、肥胖运动组;中间组、中间运动组;抵抗组、抵抗运动组。各组继续进行高脂饮食,运动组同时进行游泳运动训练。干预7周后,测定血浆NPY、胰腺NPY受体基因表达等指标。结果:(1)肥胖组体重、脂肪量、脂体比、总摄食量显著高于中间组和抵抗组;抵抗组体重和脂肪量显著低于中间组。(2)肥胖运动组与肥胖组相比,体重、脂肪量、脂体比显著下降;中间运动组与中间组相比,体重显著降低。抵抗运动组与抵抗组相比,体重、脂体比没有显著变化。(3)肥胖组血浆NPY显著高于中间组和抵抗组(P<0.01);抵抗组血浆NPY显著低于中间组(P<0.05)。肥胖运动组与肥胖组相比,血浆NPY显著下降(P<0.05)。中间运动组与中间组、抵抗运动组与抵抗组相比没有显著变化。肥胖组与抵抗组相比,胰腺NPY Y1受体基因表达没有显著差异;肥胖组和抵抗组胰腺NPY Y1受体基因表达显著低于中间组(P<0.01)。肥胖运动组与肥胖组相比,NPY Y1受体基因表达显著升高(P<0.01)。中间运动组与中间组相比没有显著变化。抵抗运动组与抵抗组相比,NPY Y1受体基因表达也显著升高(P<0.01)。结论:(1)应用高脂膳食成功复制肥胖和肥胖抵抗模型,肥胖大鼠与肥胖抵抗大鼠相比,存在脂代谢紊乱和NPY抵抗。(2)在高脂膳食喂养过程中,胰腺NPY受体表达下调,NPY敏感性下降,出现NPY抵抗,促使肥胖的发生发展。(3)长时间有氧运动能有效增加胰腺的NPY受体表达,改善机体NPY抵抗,维持机体能量代谢平衡。
To develop a rat model of diet-induced obesity and resistance by high fat diets,and to study the effect of aerobic exercise on the expression of Neuropeptide Y receptors mRNA expression in pancreas.Methods：1） 80 male SD rats were fed high fat diets for 8 weeks.The diet-induced obese rats and diet-induced resistant rats were selected based on the body weight.2） The model group was randomly divided into 6 groups：obese group,obese-exercise group,middle group,middle-exercise group,the resistant group,resistant-exercise group.All the rats were continually given high fat diets;exercise group accepted swimming training at the same time.3） After 7 week-interventions,the plasma NPY and the expression of NPY receptor in pancreas were determined.Results：1） At the end of experiment,compared with the middle group and resistant group and obese group had obviously increased body weight,fat pad weight,food intake and fat pad weight/body weight.Compared with the middle group,the resistant group had obviously reduced body weight and fat pad weight.2） Compared with obese group,obese-exercise group had obviously decreased body weight,fat pad weight,and fat pad weight/body weight.Compared with middle group,the middle-exercise group had obviously decreased body weight.Compared with resistant group,the resistant-exercise group had not shown statistical differences of body weight,fat pad weight/body weight.3） Compared with middle group and resistant group,the obese group had obviously increased plasma NPY（P〈0.01）;Compared with middle group,the resistant group had obviously decreased plasma NPY（P〈0.05）.Compared with obese group,the obese-exercise group had obviously decreased plasma NPY（P〈0.05）.Compared with middle group and the resistant group,the middle-exercise group and resistant-exercise group had not shown statistical differences.Compared with resistant group,the obese group had not show statistical differences of NPY Y1 receptor mRNA expression in pancreas.NPY Y1 receptor mRNA expression of middle group had obviously been lower than obese group and resistant group（P〈0.01）.Compared with obese group,obese-exercise group had obviously increased NPY Y1 receptor mRNA expression（P〈0.01）.Compared with the middle group,the middle-exercise group had not shown statistical differences.Compared with the resistant group,the resistant-exercise group had obviously increased NPY Y1 receptor mRNA expression（P〈0.01）.Conclusion：（1） this paper succeeds to replication a rat model of diet-induced obesity and resistance by high fat diets.Compared with the resistant rats,the obese rats have lipid metabolism disorders and NPY resistance.2） NPY receptor expression down-regulation of pancreatic in the process of the high fat diet feeding,then NPY sensitivity decline,which cause NPY resistance and weaken inhibition for insulin.As a result,the regulation relations of NPY and insulin in the pancreas are in disorder,which prompts the development of obesity.3）NPY receptor expression in the pancreas has improved through long aerobic exercise,which increased the sensitivity of NPY and improved NPY resistance,which maintains the metabolic balance of body.
出处
《沈阳体育学院学报》
CSSCI
北大核心
2012年第5期68-72,84,共6页
Journal of Shenyang Sport University
基金
辽宁省高等学校优秀人才支持项目(WR2011012)
辽宁省教育厅科研项目(W2010421)
关键词
肥胖
肥胖抵抗
有氧运动
胰腺
神经肽Y
Y1受体
diet-induced obese
diet-induced obese resistant
aerobic exercise
pancreas
Neuropeptide Y
Y1 receptor
