摘要
目的比较金樱根不同极性溶剂的提取物的抑菌作用。方法用5种不同极性的溶剂提取金樱根,用倍比稀释法和抑菌圈测定其抑菌作用。结果金樱根水提取物对金黄色葡萄球菌、绿脓杆菌、变形杆菌、伤寒杆菌的最低抑菌浓度MIC值分别为:10、40、10、40mg/ml,金樱根75%乙醇提取物对金黄色葡萄球菌、痢疾杆菌、变形杆菌、伤寒杆菌的最低抑菌浓度MIC值分别为:10、10、10、20 mg/ml,金樱根乙酸乙酯提取物对金黄色葡萄球菌的MIC值为20 mg/ml,对痢疾杆菌、变形杆菌、伤寒杆菌无明显抑制作用;石油醚提取物和氯仿提取物无抑菌作用。结论金樱根的水提取物和乙醇提取物有较好抑菌作用,其它溶剂提取物抑菌作用较差。
Objective To compare the different antibacterial effect of the extractive of Radix Rosae Laevigatae （RRL） by different polar solvents. Methods Five different polar solvents were used to extract RRL, and doubling dilution method and inhibition zone were used to measure the inhibitory effect. Results The MIC values of water extract of RRL againsts Staphylococcus aureus （ S. aureus）, Pseudomonas aeruginosa （ P. aeruginosa）, Proteus vulgaris （ P. vulgaris ）, Salmonella （SA） typhi were 10,40,10,40 mg/ml, the MIC inhibitory concentration values of 75 % alcohol extract of RRL against S. aureus, Shigella Castellani and Chalmers （S. C）, P. vulgaris, SA typhi were 10,10,10,20 mg/ml, the MIC value of ethyl acetate extract of RRL against only S. aureus is 20 mg/ml. Petroleum ether extract and chloroform extract of RRL haven＇t antibacterial effect. Conclusion The water extract and alcohol extract of RRL have good antibacterial effects, the antibacterial effect of ethyl acetate extract is less, petroleum ether extract and chloroform extract of RRL have inhibitory effect.
出处
《今日药学》
CAS
2012年第9期531-533,共3页
Pharmacy Today
基金
中山市健康基地专项基金(编号:2009H020)
关键词
金樱根
提取物
体外抑菌
最低抑菌浓度
Radix Rosae laevigatae
extract
in vitro bacteriostasis
inhibitory concentration
