摘要
为研究白头翁皂苷体外抗肿瘤活性,从白头翁中提取有效成分皂苷,对大肠癌HT29细胞和人胃癌细胞株BGC823进行体外培养,用MTT法测定白头翁皂苷对2种肿瘤细胞增殖反应的影响。结果表明:白头翁皂苷对两种肿瘤细胞增殖有较强的抑制作用,并表现一定的剂量关系,白头翁皂苷浓度为6μg.mL-1时,对人胃癌细胞株BGC823细胞生长的抑制率达到了80.71%,效果较好。因此,白头翁皂苷作为抗肿瘤药物有效成分的开发和利用有待于进一步深入研究。
To study the anti-tumor activity of Pulsatilla saponin in vitro,as the effective components,the Pulsatilla saponin was extracted from Pulsatilla chinensis to culture the colorectal carcinoma HT29 cells and human gastric carcinoma cell line BGC823 in vitro,the effect of Pulsatilla saponin on two kinds of tumor cell proliferation was determined by the method of MTT.The results showed that the Pulsatilla saponin had strong inhibition on two kinds of tumor cells proliferation,and the inhibition enhanced with the increasing of concentration of Pulsatilla saponin,the inhibition rate on BGC823 cell growth reached 80.71% when the concentration was 6 μg·mL-1.Therefore,it needs further deeply study on the development and utilization of the Pulsatilla saponin as anti-tumor drug effective components.
出处
《黑龙江农业科学》
2012年第10期125-127,共3页
Heilongjiang Agricultural Sciences
基金
黑龙江省卫生厅科研资助项目(2011-455)
