摘要
目的:对比分析新疆维、哈两民族共411人ApoE等位基因多态性与冠心病相关性;同时进行等位基因频率的对比分析。方法:利用酚-氯仿法抽提提取DNA,再将PCR产物进行纯化。SNaPshot多重单碱基延伸反应纯化后得到的延伸产物在ABI3130XL基因分析仪上进行测序。结果:维、哈两民族对照组与冠心病组之间,冠心病与冠心病组之间E2、E3、E4各单倍体,各基因型阳性率居于相同水平P>0.05)。结论:新疆维、哈两民族ApoE等位基因阳性率水平无统计学差异。
Objective：To explore the relationship of ApoE gene polymorphism and coronary heart disease and compare the frequency of alleles in nationality of Uygurs and Kazaks.Methods：411 cases were studied.DNA product was extracted by using the Phenol-chloroform method and the PCR outcome was purified.We take advantage of Multiple single base extension reaction to make DNA sequencing on the ABI3130XL analysis meter.Results：The E2,E3,E4 various haploids,various genotypes masculine gender rate resides were in the same level（P0.05） between the control groups and coronary disease group;and between the coronary disease groups in two nationality of Uygur and Kazak.Conclusion： ApoE allele masculine gender rate level is non-statistics difference in two nationality of Uygur and Kazak in Xinjiang.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2012年第9期807-809,共3页
Chinese Journal of Immunology
基金
新疆维吾尔自治区科技支疆项目(No.01091141)
关键词
新疆维、哈民族
ApoE等位基因多态性
冠心病
血脂
Xinjiang Uighurs and Kazakh
ApoE gene polymorphism
Coronary heart disease
Blood-lipid
