摘要
目的:建立气阴两虚、瘀血阻络型糖尿病合并局灶性脑缺血大鼠模型,观察具有益气养阴活血功效的参芎玉精颗粒对模型大鼠海马齿状回区神经细胞的增殖、分化和存活的影响。方法:将大鼠分为假手术组、糖尿病合并脑缺血(气阴两虚、瘀血阻络证)模型对照组、参芎玉精组和参芎组,每组20只,采用5-溴-2′-脱氧尿嘧啶掺入法、免疫组织化学法观察参芎玉精颗粒对糖尿病合并脑缺血大鼠神经细胞增殖、分化和存活的影响。结果:糖尿病合并脑缺血可使大鼠海马齿状回颗粒下层的新生细胞增多,参芎玉精颗粒作用7d时不能促进糖尿病合并脑缺血大鼠颗粒下层新生细胞的增殖(P>0.05),但治疗21d后,可以明显提高新生细胞的存活率,促进新生细胞向神经元分化(P<0.01),并优于参芎颗粒(P<0.01)。结论:以益气养阴活血法组方的参芎玉精颗粒可促进气阴两虚、瘀血阻络型糖尿病合并脑缺血大鼠新生神经细胞的存活,并促进其向神经元分化,效果优于益气活血法。
To establish a rat model of diabetes-associated cerebral ischemia due to qi and yin deficiency and blood stasis, and to investigate the effects of Radix Ginseng, Rhizoma Chuanxiong, Rhizoma Polygonati Odorati and Rhizoma Polygonati Sibirici Granule （Shenxiong Yujing Granule）, which has the function of strengthening qi, nourishing yin, and activating blood, on proliferation, differentiation and survival of neural cells in rats with diabetes-associated cerebral ischemia. METHODS： Rats were divided into sham-operation, diabetes plus ischemia reperfusion injury model, Shenxiong Yujing Granule and Radix Ginseng and Rhizoma Chuanxiong Granule （Shenxiong Granule） groups with 20 rats in each. The 5-bromo-2＇-deoxyuridine （BrdU） incorporation assay and immunohistochemical method were used to investigate the proliferation, differentiation and survival of neural cells in dentate gyrus of rats with diabetes-associated cerebral ischemia. RESULTS： The number of newly proliferating cells in subgranular zone of dentate gyrus was increased in the model group, but there was no significant difference compared with 7 day treatment with Shenxiong Yujing Granule. Shenxiong Yujing Granule significantly increased the survival rate and promoted the differentiation of newly proliferating neurons after 21-day treatment （P〈0.01）. In addition, the beneficial effect of Shenxiong Yujing Granule was considerably greater than that of the Shenxiong Granule （P〈0.01）. CONCLUSION： Shenxiong Yujing Granule can increase the survival rate and promote the differentiation of newly proliferating neurons in rats with diabetes-associated cerebral ischemia of dual deficiency of qi and yin and blood stasis obstructing the collaterals. The effect is greater than that of Shenxiong Granule.
出处
《中西医结合学报》
CAS
2012年第10期1140-1148,共9页
Journal of Chinese Integrative Medicine
基金
国家自然科学基金资助项目(No.81001519
No.81173210)
安徽省高校省级自然科学研究重点项目(No.KJ2010A207)
安徽中医学院第一附属医院国家中医药重点学科中医内分泌学学科开放研究课题资助项目(No.2011nfmxk003)
安徽中医学院第一附属医院国家中医临床研究基地重点病种糖尿病院内课题资助项目(No.2009LC1-011ZX)
关键词
中草药
糖尿病
脑缺血
神经再生
神经元
疾病模型
动物
星形细胞
大鼠
drugs, Chinese herbal
diabetes mellitus
brain ischemia
nerve regeneration
neurons
diseasemodels, animal
astrocytes
rats
