摘要
目的观察三物白散药物血清对人胃癌SGC-7901细胞凋亡及survivin mRNA表达的影响。方法选择人胃癌细胞株SGC-7901,分为空白对照组、阳性对照组及三物白散低、中、高剂量组。三物白散药物血清干预SGC-7901细胞后,采用荧光染色法检测胃癌细胞的凋亡率,RT-PCR检测survivin mRNA的表达。结果不同剂量组的三物白散药物血清干预SGC-7901细胞后,与空白对照组比较,各组细胞的凋亡率升高,且细胞凋亡率随药物血清浓度的增大、干预时间的延长逐渐升高;三物白散各剂量组survivin mRNA的表达均有不同程度的下降,与空白对照组比较,差异均有统计学意义(P<0.05),且survivin mRNA表达随三物白散含药血清浓度的增大、干预时间的延长逐渐下降。结论三物白散可诱导胃癌SGC-7901细胞凋亡,抑制survivin mRNA的表达。
Objective To investigate the effect of Sanwubaisan-containing serum on the apoptosis and survivin expression of human gastric cancer cell line SGC-7901.Methods Human gastric cancer cell line SGC-7901 cells were divided into normal group,low-dose Sanwubaisan group,medium-dose Sanwubaisan group,high-dose Sanwubaisan group,positive control group.The apoptosis was measured by fluorescence staining after treated by Sanwubaisan-containing serum,survivin mRNA expression was detected by RT-PCR.Results After the SGC-7901 cells treated by different concentrations of Sanwubaisan-containing serum,compared with the normal group,there was a increasing tendency on the apoptosis of SGC-7901 cells,depending on the concentration and time.There was a decreasing tendency in different degrees on the survivin mRNA expression after the SGC-7901 cells treated by different concentrations of Sanwubaisan-containing serum,with statistical significance（P0.05） compared with the normal group,and decreased with the concentration and time.Conclusion Sanwubaisan-containing serum can induce apoptosis of SGC-7901 cells and inhibit survivin mRNA expression of gastric cancer SGC-7901 cells.
出处
《中国中医药信息杂志》
CAS
CSCD
2012年第10期35-37,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
湖南省教育厅重点项目(09A069)
