摘要
目的建立同时测定陇中损伤胶囊中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1含量的高效液相色谱梯度洗脱方法。方法采用C18柱(250 mm×4.6 mm,5μm);流动相为乙腈(A)-0.05%磷酸溶液(B);梯度洗脱0～32 min(A23%～23%),32～42 min(A 23%～30%),42～67 min(A 30%～46%);检测波长203 nm;流速1.0 mL/min。结果三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1进样量分别在1.02～8.50μg(r=0.9995)、0.90～7.50μg(r=0.9990)、1.17～9.75μg(r=0.999 6)范围内与峰面积线性关系良好,平均加样回收率为100.98%(RSD=2.71%)、97.73%(RSD=1.98%)、99.57%(RSD=1.62%)。结论所用方法简单易行、准确可靠,可作为陇中损伤胶囊的质量控制方法。
Objective To establish the method for simultaneous determination of panax notoginseng saponins R 1,ginseng saponins Rg 1,ginseng saponins Rb 1 in Longzhong Sunshang Capsule by HPLC with gradient elution.Methods C 18 column（250 nm×4.6 nm,5 μm） was used with mobile phase of acetonitrile（A）-0.05% phosphoric acid solution（B）,gradient elution 0-32 min（A 23%-23%）,32-42 min（A 23%30%）,42-67 min（A 30%-46%）.The detection wavelength was 203 nm,and the flow rate was 1.0 mL/min.Results The linear relationship of panax notoginseng saponins R 1,ginseng saponins Rg 1,ginseng saponins Rb 1 was in the range of 1.02-8.50 μg（r =0.999 5）,0.90-7.50 μg（r =0.999 0） and 1.17-9.75 μg（r = 0.999 6） respectively.The average recovery was 100.98%（RSD=2.71%）,97.73%（RSD=1.98%） and 99.57%（RSD=1.62%）.Conclusion The method is simple,accurate,reliable,and can be used for quality control of Longzhong Sunshang Capsule.
出处
《中国中医药信息杂志》
CAS
CSCD
2012年第10期41-43,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
兰州市科技局立项课题(2010-1-161)
关键词
陇中损伤胶囊
三七皂苷R1
人参皂苷RG1
人参皂苷RB1
含量测定
Longzhong Sunshang Capsule
panax notoginseng saponins R 1
Ginseng saponins Rg 1
Ginseng saponins Rb 1
content determination
