摘要
对兰花组织培养的历史和进展作了扼要综述 .着重阐述了外植体取材、培养基、激素等因素对外植体培养、原球茎增殖及芽分化的影响 ,并介绍了兰花种子离体萌发技术、兰花原生质体培养。
The history and development of study on tissue culture of orchid are summerized.Effects of explants,media,growth regulators on culture of explant,multiplication of PLBs,differentiation of shoots and the seed germination of orchid in vitro,and the progress on orchid protoplast culture,protoplast fusion,orchid genetic engineering are introduced.
出处
《西北师范大学学报（自然科学版）》
CAS
2000年第3期111-116,共6页
Journal of Northwest Normal University(Natural Science)
关键词
组织培养
外植体
基因工程
兰花
生物工程
兰科
tissue culture
explant
PLB
medium
germination in vitro
genetic engineering
