摘要
目的了解不同储存时间的悬浮红细胞(悬红)制备的洗涤红细胞中钾离子浓度([K+])和游离血红蛋白含量(FHb)。方法将储存3 d(3 d组,n=6)、16 d(16 d组,n=6)及31～35 d(31～35 d组,n=7)的悬红,手工制备成洗涤红细胞,分别于制备前、制备后0、16和24 h取样测定其[K+]和FHb。结果 3 d组、16 d组和31～35d组悬红制备的洗涤红细胞24 h时[K+]分别为(3.26±0.57)、(3.39±1.12)及(2.97±0.95)mmol/L(P>0.05),且均低于人体正常[K+]上限;3组制备的洗涤红细胞24 h时的FHb分别为(0.29±0.18)、(0.63±0.40)及(1.06±0.55)g/L,其中31～35 d组制备的洗涤红细胞的FHb明显高于3 d组(P<0.01)。结论悬红储存时间对制备的洗涤红细胞的[K+]影响不大,制备的洗涤红细胞24 h保存期内的[K+]均低于人体正常参考值上限,但对FHb含量有较大影响。建议尽量避免使用接近储存期末的悬红制备洗涤红细胞。
Objective To study the influence of the storage time of red blood cells （RBCs） on the concentrations of potassium （ [ K ＋ ] ） and free hemoglobin （FHb） of prepared washed red blood cells （ washed RBCs）. Methods RBCs preserved for 3 d （3 d group,n =6） ,16 d （16 d group,n =6） and 31 -35 d （31 35 d greup,n =7） were chosen to pre- pare washed RBCs manually,and samples were taken at the time before preparation,0,16 and 24 h after preparation, re- spectively. [K＋ ] and FHb were measured. Results The [K~ ] of washed RBCs 24 h after preparation of 3 d group,16 d group and 31 ~35 d group were （3.26 ~0. 57）, （3.39 ~ 1.12） and （2.97 ~0. 95） retooL/L, respectively （P 〉0. 05）, and all the [ K ＋ ] were still lower than the upper limit of [ K ＋ ] in normal human bodies. The FHb of washed RBCs 24 h af- ter preparation of these three groups were （0. 29 ~ 0. 18）, （0. 63 ~ 0. 40） and （ 1.06 ~ 0. 55 ） g/L, respectively. The FHb of washed RBCs 24 h after preparation of 31 ~ 35 d group was significantly higher than that of 3 d group （P 〈 0. 01 ） ~ Conclusion There is little influence of the storage time of RBCs on the [ K ＋ ] of prepared washed RBCs, with the [ K ＋ ] of all prepared washed RBCs lower than the upper limit of [ K＋] in normal human bodies. However,the storage time of RBCs affects the FHb of prepared washed RBCs significantly, so RBCs close to the end of storage should be avoided to pre- pare washed RBCs.
出处
《中国输血杂志》
CAS
CSCD
北大核心
2012年第9期834-836,共3页
Chinese Journal of Blood Transfusion
基金
卫生部卫生行业科研专项(201002005)
关键词
悬浮红细胞
储存时间
洗涤红细胞
钾离子浓度
游离血红蛋白
Red blood cells
Storage time
Washed red blood cells
Concentrations of potassium
Free hemoglobin
