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Detection of Penicillin via Surface Plasmon Resonance Biosensor 被引量:2

Detection of Penicillin via Surface Plasmon Resonance Biosensor
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摘要 A method of using Au colloid to capture the decomposed product of penicillin, penicillamine, on a surface plasmon resonance(SPR) biosensor for the quantitative determination of penicillin was developed. Based on the de-composition of penicillin to generate penicillamine and penilloaldehyde, a high sensitive biosensor for detecting penicillin was also developed. In our experiment, it was penicillamine rather than penicillin that has been measured. This is because penicillamine contains a functional group that makes it self-assembling on Au colloid to increase the molecular weight so as to improve the surface plasmon resonance signal. On a UV-Vis spectrophotometer, a high concentration of penicilliamine-Au complex was determined, indicating that penicillamine was already well combined with Au colloid. The method, using the combination of Au colloid with penicillamine, proved to detect penicillin. A method of using Au colloid to capture the decomposed product of penicillin, penicillamine, on a surface plasmon resonance(SPR) biosensor for the quantitative determination of penicillin was developed. Based on the de-composition of penicillin to generate penicillamine and penilloaldehyde, a high sensitive biosensor for detecting penicillin was also developed. In our experiment, it was penicillamine rather than penicillin that has been measured. This is because penicillamine contains a functional group that makes it self-assembling on Au colloid to increase the molecular weight so as to improve the surface plasmon resonance signal. On a UV-Vis spectrophotometer, a high concentration of penicilliamine-Au complex was determined, indicating that penicillamine was already well combined with Au colloid. The method, using the combination of Au colloid with penicillamine, proved to detect penicillin.
出处 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2012年第5期792-796,共5页 高等学校化学研究(英文版)
基金 Supported by the National Natural Science Foundation of China(No.31070772) the Science and Technology Programs of Zhejiang Province, China(No.2011C37029) the Doctoral Program of Higher Education of China(No.200901011110136) the Science and Technology Programs of Suzhou City, China(No.ZXG0920)
关键词 Surface plasmon resonance(SPR) BIOSENSOR PENICILLAMINE Au colloid Surface plasmon resonance(SPR) Biosensor Penicillamine Au colloid
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