摘要
目的:探讨脑脊液中16SrRNA基因检测在新生儿化脓性脑膜炎早期诊断中的应用价值。方法:对疑似化脓性脑膜炎的40例患儿的脑脊液进行16SrRNA基因PCR检测,并同时做脑脊液常规、生化及细菌培养,将其结果进行比较。结果:40例患儿脑脊液16SrRNA基因PCR检测阳性率32.5%(13/40),脑脊液细菌培养阳性率0%(0/40)。根据脑脊液常规、生化检测参数临床诊断为化脓性脑膜炎的患者为12.5%(5/40)。16SrRNA基因PCR检测阳性率明显高于脑脊液培养和临床诊断(P<0.05)。结论:16SrRNA基因PCR检测方法特异性强、敏感性高,可为临床新生儿化脓性脑膜炎的早期诊断提供可靠的依据。
Objective：To explore the meaning PCR detection of 16SrRNA in the neonatal purulent menin- gitis. Methods：40 samples with suspect neonatal purulent meningitis was collected. Every sample was detected with routine test,culture and PCR. Results：The positive rate was 0% of CSF culture and 32.5% of PCR. The outcome has statistical significance. Conclusion：PCR with high sensitivity and specificity can improve the positive rate,worthy of clinical promotion.
出处
《陕西医学杂志》
CAS
2012年第10期1389-1390,共2页
Shaanxi Medical Journal
