摘要
基于报道的大豆干种子提取方法,对高脂肪的杏仁进行了基因组DNA提取方法的改良研究,并以提取的基因组为模板进行了PCR扩增验证。结果表明:改良的SDS法提取的杏仁基因组DNA具有纯度和浓度高、条带清晰、降解少等优点,并在高倍稀释条件下仍能获得有效扩增。
Based on the genomic extraction method reported in soybean seeds,the improved method was used for genomic DNA isolation of high fat almond,and the genome extracted as a template for the PCR amplification verification.The results showed that the DNA extractioned in improved SDS method had these advantages：purity and concentration was high,the strip was clear,degradation was less,and in high dilution conditions could still obtain effective amplification.
出处
《北方园艺》
CAS
北大核心
2012年第20期105-107,共3页
Northern Horticulture
