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CRISPR系统结构与功能研究进展 被引量:3

Progress on Structure and Function of CRISPR System
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摘要 自然界中的微生物总是通过各种各样的保护机制,使它们能够与恶劣的环境及侵袭性核酸共存。许多微生物都能通过基于基因序列特异性的方式抵抗核酸入侵。CRISPR(clustered regularly inter-spaced short palindromic repeats)即是属于这种保护机制之一。该位点是由独特的DNA间区间隔开的短的重复序列组成,并与一些被称为CRISPR相关蛋白(CRISPR association protein)的蛋白质共同作用,形成一套完整的自我保护机制。这种高变位点能从外源片段中摄取约36bp的短片段,从而建立可遗传的、DNA介导的"特异性免疫保护机制"。 Bacteria can protect them from bad environment and invading nucleic acid by various protection mechanisms in nature. Most bacteria have the self-defense mechanisms mediated by specific genes. A genetic locus termed CRISPR (clustered regularly interspaced short palindromic repeats) is exit in many bacteria and archaea, which can provide bacteria from plasmid and invading acid though sequence-specificity. CRISPR consists of short repeat interspersed with unique DNA spacers, and adjacent CRISPR-associated protein encoding genes. CRISPR loci can take up foreign nucleic acid elements to build the genetic and DNA-mediated "specific immune protection mechanism".
出处 《动物医学进展》 CSCD 北大核心 2012年第10期82-86,共5页 Progress In Veterinary Medicine
基金 湖南省科技计划项目(2010NK3015)
关键词 CRISPR位点 噬菌体 质粒 外源基因片段 CRISPR-loc bacterophage plasmid foreign gene element
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