摘要
目的:观察染料木素(Gen)对异丙肾上腺素(Iso)所致心肌肥厚大鼠的抗氧化及抑制炎症反应作用。方法:采用背部sc给予Iso 1 mg.kg-1.d-1,连续10 d,建立大鼠心肌肥厚模型。造模第2天,正常对照组及模型组于背部sc给予NS2 mL.kg-1.d-1,溶剂对照组给予等体积7%DMSO,Gen组给予等体积的Gen 0.03,0.1,0.3μmol.kg-1,连续14 d。末次给药后禁食12 h,称体重,麻醉,取静脉血,分离血清,取心脏,称左心室质量,计算左心室质量参数;检测血清超氧化物歧化酶(superoxide dismutase,SOD)、髓过氧化物酶(myeloperoxidase,MPO)活性及丙二醛(malondialdehyde,MDA)含量;放射免疫学方法检测心肌组织白细胞介素2(interleukin-2,IL-2)含量。结果:与正常对照组相比,模型组大鼠左心室质量参数明显升高(2.48±0.11)mg.kg-1,P<0.001,血清SOD活性明显降低(128.19±11.20)U.mL-1,P<0.05,血清MDA含量增高(15.81±1.96)mmol.mL-1,P<0.05,血清MPO活性提高(12.22±2.34)U.mL-1,P<0.05,心肌组织IL-2含量增高(11.41±1.08)pg.mgprot-1,P<0.05。染料木素低、中、高剂量明显提高血清SOD活性(160.47±16.01,164.36±9.39,172.03±8.42)U.mL-1,P<0.01,降低血清MDA含量(11.96±2.17,11.72±0.73,10.93±0.52)mmol.mL-1,P<0.05,降低血清MPO活性(9.84±2.03,8.91±2.03,7.51±1.97)U.mL-1,P<0.01,降低心肌组织IL-2含量(9.70±1.81,5.95±3.39,6.33±1.19)pg.mg prot-1,P<0.05。结论:染料木素可通过提高抗氧化能力及抗炎症反应抑制Iso诱导的大鼠心肌肥厚。
Objective：To observe the antioxidation and anti-inflammation effect of genistein（Gen） on rat myocardial hypertrophy caused by isopropylarterenol（Iso）.Method：The rat myocardial hypertrophy model was reproduced through hypodermic injection which lasted 10 days at back skin with Iso,1 mg · kg-1 · d-1.Once the model was succesfully made,rats were administrated with corresponding doses of Gen,DMSO or normal saline through hypodermic injection from the second day,and the process lasted 14 days.After the last administration,the rats were lasted overnight and samples of blood and heart were collected under anesthesia status.The indexes of left ventricle were calculated,the activity of superoxide dismutase（SOD）,myeloperoxidase（MPO） and the concentration of malondialdehyde（MDA） in serum were detected,and interleukin-2（IL-2） in myocardial tissue was investigated with radioimmunity kits.Result：In model group,there was a marked increase in the left ventricle index,the activity of SOD in the serum was markedly decreased,the content of MDA and the activity of MPO in the serum were increased,the level of IL-2 in myocardial tissue of rats was increased.Treatment with Gen significantly increased the activity of SOD in the serum,decreased the content of MDA and the activity of MPO in the serum,reduced the level of IL-2 in myocardial tissue.Conclusion：Gen has antioxidation and anti-inflammation effect on the myocardial hypertrophy caused by Iso.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第19期175-178,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江西省卫生厅科技项目(20093191)
关键词
染料木素
异丙肾上腺素
心肌肥厚
抗氧化作用
抗炎症反应
genistein
isopropylarterenol
myocardial hypertrophy
antioxidation
anti-inflammation effect
