摘要
目的:建立测定盐酸多巴酚丁胺注射液中亚硫酸氢钠含量的抑制型离子色谱方法。方法:采用IonPacAG11-HC(250mm×4.0 mm)色谱柱,以30 mmol·L-1氢氧化钠为淋洗液,流速为1.0 mL·min-1,抑制器电流为100 mA。结果:亚硫酸氢钠在1.0～10.0 mg·L-1(r=0.9997)范围内有良好的线性响应关系,平均回收率(n=9)为98.7%,RSD为1.5%,最低检出限为0.05 mg·L-1。结论:本方法准确、简便、快速,可用于注射液中亚硫酸氢钠的快速测定。
Objective：To establish a suppressed conductive ion chromatography method for the determination of sodium hydrosulfite in dobutamine hydrochloride injection.Methods：Chromatographic separation was achieved on an IonPacAG11-HC（250 mm×4.0 mm） column with 30 mmol·L-1NaOH elution at the flow rate of 1.0 mL·min-1.The suppressor was operated at a current of 100 mA.Results：The linear range of sodium hydrosulfite was 1.0-10.0 μg·mL-1（r=0.9997）.The average recovery was 98.7% with an RSD of 1.5%（n=9）.The detection limit was 0.05 mg·L-1.Conclusion：The method is accurate,convenient and rapid,which provides a reliable way for the determination of sodium hydrosulfite in injection.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2012年第10期1835-1837,1830,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
离子色谱
亚硫酸氢钠
盐酸多巴酚丁胺
注射剂抗氧剂
快速测定
ion chromatography（IC）
sodium hydrosulfite
dobutamine hydrochloride
injection antioxidant
rapid determination
