摘要
目的:建立去皮与不去皮桔梗饮片鉴别方法。方法:采用TLC和HPLC,对去皮与未去皮桔梗饮片进行定性鉴别。结果:两种桔梗饮片的TLC图谱及HPLC图谱差异显著,未去皮桔梗饮片比去皮桔梗饮片多出3个斑点;HPLC图谱显示两种饮片均含有13个共有峰,但未去皮桔梗比去皮桔梗多出5个色谱峰,可以用于两种饮片的鉴别。结论:建立的TLC和HPLC图谱鉴别方法简便易行,可有效地区分去皮与未去皮桔梗药材和饮片,丰富了桔梗饮片的质量评价方法。
Objective：To establish a new method to identify the peeled and non-peeled pieces of Platyeodon grandiflorum. Method： TLC and HPLC chromatography were used to identification. Result： There have significant differences in two kinds of pieces. The non-peeled pieces have three extra spots more than the peeled pieces and the HPLC chromatography showed five extra peaks expect thirteen common peaks. Conclusion：The established method was simple, it could be used for evaluation of different pieces of P. grandiflorum more effectively. Meanwhile, it also enriches the quality evaluation method.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第20期66-68,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家中医药管理局中医药科学技术研究专项(06-07ZQ20)
国家科技重大专项项目(2009ZX09301-005-03)
关键词
桔梗
去皮饮片
不去皮饮片
色谱鉴别
Platyeodon grandiflorum
peeled pieces
unpeeled pieces
chromatographic identification
