摘要
为了优化橡胶树内珠被植株再生体系,促进其次生体胚技术和转基因研究的发展,以橡胶树品种‘热研7-33-97’和‘热研88-13’的幼果内珠被为材料,进行不同因素对橡胶树内珠被愈伤组织诱导和体细胞胚发生的研究。在添加不同浓度的2,4-D,外加80g/L蔗糖、50mg/L椰子水、5.0mg/L AgNO3和2.3g/L phytagel的MH培养基上诱导愈伤组织的产生。结果表明,当培养基中不添加2,4-D时,2个品种均不能诱导出愈伤组织,随着2,4-D浓度的增加,其愈伤诱导率不断增加,达到4.0mg/L水平时,诱导率均达到最大,分别为88.28%、86.67%。当浓度逐步升高至6.0mg/L时,其诱导率又有所下降。愈伤诱导后,将其转移到添加了不同浓度NAA的改良MS基本培养基上。结果发现,0.2mg/L的NAA浓度更有利于橡胶树‘热研7-33-97’内珠被子叶胚、喇叭胚、杯状胚和多叶胚等胚的产生,但子叶胚出胚率也仅达4.00%,而低于或高于此浓度均不利于体细胞胚的产生。
In order to optimize the plant regeneration system of inner integument and promote the development of secondary somatic embryogenesis and transgenetic research of Hevea brasiliensis.Callus induction and somatic embryogenesis were conducted on different factors by taking inner integument of immature fruits from two clones＇Reyan 7-33-97＇and＇Reyan 88-13＇of Hevea brasiliensis.Calla induction were obtained on MH medium supplemented with 80 g/L sugar,50 mg/L coconut water,5.0 mg/L silver nitrate,2.3 g/L phytagel and different concentrations of 2,4-D.The results showed that callus could not be inducted from two clones when the media had no 2,4-D.The induction rate of callus was gradually improved with the increasing of 2,4-D concentration.The rate was the highest,while the concentration reached 4.0 mg/L,respectively 88.28% and 86.67%.When the concentration of 2,4-D reached 6.0 mg/L,the rate was gradually declining.After theinduction of callus,callus was transferred to modified MS medium supplemented with different concentrations of NAA.The results indicated that the embryoids of cotyledonary,cup-like,trumpet-like and leafy were easily produced with the concentration of 0.2 mg/L NAA from the inner integument of＇Reyan 7-33-97＇.The induction rate of cotyledonary embryoid reached only 4.00%.Somatic embryo was not beneficial to be produced when the concentration of NAA was lower or more than 0.2 mg/L.
出处
《中国农学通报》
CSCD
2012年第25期15-19,共5页
Chinese Agricultural Science Bulletin
基金
海南省自然科学资金"橡胶树内珠被愈伤组织继代培养及其RAPD分析"(809028)
中国热带农业科学院橡胶研究所基本科研业务费专项项目"橡胶树次级体细胞胚发生的分子机理研究"(1630022011004)
关键词
橡胶树
内珠被
愈伤组织
体细胞胚发生
激素
Hevea brasiliensis
inner integument
callus
somatic embryogenesis
plant hormone
