摘要
目的建立超高效液相色谱法同时测定化妆品中9种邻苯二甲酸酯和双酚A的分析方法。方法样品经甲醇提取后,用超高效液相色谱带PDA检测器进行检测,色谱柱:苯基柱(50mm×2.1mm i.d.,1.7μm)。定量检测波长为275nm。结果 9种邻苯二甲酸酯的线性范围为10.0～300.0μg/ml,双酚A线性范围为5.0～150.0μg/ml;相关系数r≥0.9996;邻苯二甲酸二甲酯和邻苯二甲酸二乙酯的检出限为0.4μg/ml,双酚A的检出限为0.2μg/ml,邻苯二甲酸二丙酯、邻苯二甲酸二异丁酯、邻苯二甲酸二丁酯、邻苯二甲酸丁基苄酯、邻苯二甲酸二戊酯、邻苯二甲酸二己酯和邻苯二甲酸双(2-乙基己基)酯的检出限为0.8μg/ml;高浓度加标平均回收率在91.9%～101.3%,低浓度加标平均回收率在92.8%～105.4%;相对标准偏差小于5.23%。结论该方法操作简单、快速、有效,适合化妆品中9种邻苯二甲酸甲酯和双酚A的日常检测。
Objective An ultra-performance liquid chromatography methqd has been established for the simultaneous determination of 9 phthalate esters and bisphenol A in cosmetics. Methods The samples were extracted by methanol and determined by ultra- high performance liquid chromatography with PDA detector. The separation of 9 phthalate esters and bisphenol A was carried out by an Phenyl column （50mm χ 2. l mm id, 1.7 μm） column and quantification was based on detection wavelength at 275 nm. Results The linear ranges of the calibration curves for 9 phthalate esters and bisphenol A were 10-300μg/ml and 5-150μg/ml, respectively. The correlation coefficients of the standard curves were all higher than 0. 9996. The limits of detection （LODs） for phthalate esters were 0.4-0.8μg/ml and bisphenol A was 0.21μg/ml. The average recoveries at the concentrations studied were in the range from 91.9% to 105.4%. Relative standard deviations were all less than 5.23%. Conclusion The method is simple, fast and effective, and could meet the needs for routine analysis of the 9 phthalate esters and bisphenol A in cosmetics.
出处
《卫生研究》
CAS
CSCD
北大核心
2012年第5期846-849,共4页
Journal of Hygiene Research
关键词
邻苯二甲酸酯
双酚A化妆品
超高效液相色谱
phthalate esters, bisphenol A, cosmetics, ultra-high performanceliquid chromatography
