摘要
目的通过观察扶正抑癌方含药血清对结肠癌Lovo细胞TGF-β/smad信号转导通路中TGF-β1、Smad3和p-Smad3蛋白表达的影响,明确扶正抑癌方抑制结肠癌细胞间质化转变的作用机制。方法 Lovo细胞体外培养,制备大鼠不同浓度含药血清后,MTT法测定不同浓度含药血清对细胞增殖的影响,以5-Fu作为阳性对照;通过Western blot检测TGF-β1、Smad3和p-Smad3蛋白的表达。结果大剂量含药血清组24 h对Lovo细胞抑制率较中剂量、小剂量组作用更加明显,差异具有统计学意义(P<0.01)。Western blot检测结果表明扶正抑癌方含药血清可以下调Lovo细胞TGF-β1、Smad3和p-Smad3的蛋白表达,与正常组和空白组相比,差异具有统计学意义(P<0.05)。结论扶正抑癌方含药血清可能通过下调TGF-β/smad信号转导通路中TGF-β1、Smad3和p-Smad3的蛋白表达,抑制Lo-vo细胞间质化转变。
Objective To discuss the mechanism of drug serum of Fuzheng Yi＇ai decoction on epithelial- mesenchymal transition（EMT） in human colon cancer cell line Lovo by investigating the expression of TGF β1 and Smad3 and p-Smad3 protein level of TGF-β/Smad signal transduction pathway. Methods The proliferation effect on Lovo cells treated with various concentrations of drug serum was detected by MTT method. A group treated by 5-Fu was used as positive control. Then the expression of TGF-β1 and Srnad3 and p-Smad3 protein were detected by Western blot assay. Results According to the results of MTT test,the large concentrations of Fuzheng Yi＇ai decoction drug serum restrained the cells proliferation effect was obvious at 24 hours,compared with the median group and small concentration group （P〈 0. 01 ）;the Western blot result revealed that the expressions of TGF-β1 ,Smad3 and p-Smad3 protein in drug serum group were down-regulated in comparison with the blank group and normal group （P〈 0.05）. Conclusion The drug serum of Fuzheng Yi＇ai decoction can inhibit the EMT of Lovo cell correlated with the decreasing expression of TGF-β1. Smad3 and p-Smad3 protein level of TGF-β/Smad signal transduction pathway.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2012年第10期1163-1165,共3页
Cancer Research on Prevention and Treatment
基金
上海市教委重点学科建设资助项目(J50301)
