摘要
目的了解猪腹膜制作的生物护创膜覆盖微粒皮移植创面的效果。方法将20只新西兰兔按照随机数字表法分为10对，每对2只兔同时于背部左右侧对称部位分别切取1块圆形全厚皮片（直径为5cm），由此形成2个全层皮肤缺损创面，取1只兔2块全厚皮片中1块的1／5剪成0．2～0．5mm皮粒，均匀播撒于自身2个全层皮肤缺损创面（微粒皮面积：创面面积=1：10）。随后将每只免的2个创面按照随机数字表法分为实验组和对照组，分别覆盖由猪腹膜制成的生物护创膜及成对兔中，另1只兔剩余的1块异体皮。术后1～4周观察创面大体情况；术后3、4周计算创面愈合率；记录创嘶愈合时间。术后1～4周取覆盖物及创面组织行组织学观察。对数据进行配对，检验结果术后1周实验组生物护创膜与移植床贴附紧密，尤明显炎症反应；对照组异体皮成活良好。术后2周2组创面覆盖物均与创面贴附紧密，但较术后1周下燥、颜色加深。术后3周2组部分覆盖物干枯分离，其下创面愈合；术后4周2组创面基本愈合，外观无明显差异。实验组与对照组术后3剧创面愈合书分别为（92．8±6．2）％和（91．3±7．3）％，术后4周创面愈合率分别为（98．1±2．3）％和（97．0±4．6）％，组问比较差异均无统计学意义（t值分别为O．54、O．38，P值均大于0．05）。实验组与对照组创面愈合时间分别为（25．0±3．9）、（24．8±2．3）d，组问比较差异兀统计学意义（t=0．82，P〉0．05）。组织学观察显示，术后1、2周2组创面均见炎性细胞浸润及血管增生，微粒皮成活许扩增。术后3、4周2组覆盖物均逐步退变坏死、分离，其下盯见表皮细胞覆盖全部创面。结论乍物护创膜覆盖微粒皮移植创面，能够保护自体皮粒成活并逐步扩展修复创面，其效果与异体皮相当，是一种可供临床选用的新型生物材料。
Objective To study the effect of biological protective dressing made from porcine perito- neum in covering wounds witb microskin grafts. Methods Twenty New Zealand rabbits were divided into ten couples according to the random number table. Rabbils in each couple underwent surgery at the same time. A piece of full-thickness skin of 5 em in diameter was removed symmetrically from the left and right sides of the back of each rabbit, thus forming two wounds with full-thickness skin detect. One fifth of one piece of skin of one rabbit was cut into tiny pieces of 0.2-0.5 mm in size （microskin）. Then the mieroskin pieces were spread on the two wounds of the donor rabbit with the mieroskin/wound area ratio l： 10. The two wounds of each rabbil covered with microskin were divided into two groups according to the random number table. the wound was covered with biological protective dressing prepared with porcine peritoneum as experi- ment group, and the other was covered with the rest allograft in full size obtained from the other rabbit of each couple as control group. The general condition of wound was observed at post operation week （ POW ） 1-4. Wound healing rate was calculated at POW 3 and 4. Wound healing time was recorded. Specimens were harvested from wounds fur histological observation at POW 1-4. Data were processed with paired t test. Results （ 1 ） A1 POW 1 , the biological protective dressings were found to attach firmly to the wounds in ex- periment group without obvious inflammatory response; the allngrafts survived well on the wounds in control group. At POW 2, the coverings aitaehed well to the wounds of both groups, but became drier and darker as compared with those at POW 1. At POW 3, some wounds of the two groups healed when the coverings desic- cated and separated. At POW 4, all the wounds of both groups healed without obvious difference in appear-ance. （2） The wound healing rates of the experiment and control groups were respeclively （92.8 ± 6.2 ） % and （91.3± 7.3） （ t =0.54, P 〉0.05） at POW 3 and （98.1 ± 2.3）% and （97.0 ± 4.6）% （ t = 0.38, P 〉0.05） at POW 4. （3） The wound healing time was （25.0 ±3.9） d in experiment group and （24.8 ± 2.3） d in control group. The difference between them was not statistically significant （ t = 0.82, P 〉 0.05 ）. （4） Histological observation showed that wounds of the two groups were all infiltrated by inflam- matory cells, and new blood vessels were observed at POW 1 and 2. The survived mieroskin proliferated un- der the coverings. At POW 3 and 4, the coverings on the wounds of＂ two groups were gradually degenerated anti became necrotic＇ and separated from the wound beds, while the wounds underneath were re-epithelial- ized. Conclusions The effect of biological protective dressing in covering wounds grafted with microskin is as good as that of the alh, graft, as they both help the auto-microskin proliferate anti repair the wound. It could be considered to be new biological material for clinic.al application.
出处
《中华烧伤杂志》
CAS
CSCD
北大核心
2012年第5期336-340,共5页
Chinese Journal of Burns
基金
武汉市卫生局科研项目（WX12C37）
关键词
烧伤
移植
生物护创膜
微粒皮
Burns
Transplantation
Biological protective dressing
Mieroskin
