摘要
目的:观察脑缺血再灌注损伤大鼠脑红蛋白(neuroglobin,NgB)的表达以及电针预处理对NgB表达的影响,探索NgB在电针预处理诱导的脑保护效应中的作用。方法:雄性SD大鼠随机分为假手术组、模型组及电针组,每组16只。电针组给予电针刺激"百会",每天30min,持续5d。预处理后采用右侧大脑中动脉阻闭法,缺血120min后行再灌注,制备大鼠局灶性脑缺血再灌注损伤模型。脑缺血再灌后24h进行神经行为学评分,然后检测脑梗死容积,免疫荧光双标法检测NgB表达水平。另一批SD雄性大鼠随机分为假手术组、缺血再灌注6、24、72h组,每组6只,分别通过免疫荧光双标法观察缺血半暗带NgB表达水平的变化。结果:电针预处理后电针组较模型组脑梗死容积百分比明显减低(P<0.01),神经行为学评分及NgB含量明显增加(P<0.01)。脑缺血再灌注后6hNgB表达开始上调,24h为表达高峰(P<0.01),并至少可以持续到72h。结论:电针预处理能显著提高脑缺血再灌注大鼠神经行为学评分,降低脑梗死容积,并可进一步上调缺血半暗带NgB表达,诱导脑缺血耐受,从而减轻脑缺血再灌注损伤。
Objective To observe the effect of electroacupuncture （EA） preconditioning on cerebral ischemia and the role of cerebral neuroglobin （NgB） in EA-induced brain protection in focal cerebral ischemia and reperfusion injury （CI/RI） rats. Methods Male SD rats were randomly assigned to sham control, CI/RI 6 h, CI/RI 24 h and CI/RI 72 h groups （n = 6） for ob- serving changes of NgB at different time-points. Additional SD rats were randomly assigned to sham, model, and EA preconditio= ning （EA-PC） groups （n = 16） for observing changes of cerebral NgB positive cell counts in the ischemic penumbra region 24 h af- ter reperfusion. EA pre-conditioning was applied to ＂Baihui＂ （GV 20） for 30 min, once daily for 5 days before CI/Rl. CI/RI model was established by occlusion of the right middle cerebral artery and reperfusion for 6 h, 24 h and 72 h respectively. The neurologi- cal behavior scores （NBS） of all the rats were evaluated according to Garcia＇s methods. The cerebral infarct volume was deter- mined by 2,3,5-triphenyltetrazolium chloride （TTC） staining. The number of cerebral NgB positive cells was detected by immuno- fluorescent staining. Results No infarct loci were found in the sham group. The cerebral infarction volume percentage was sig- nificantly higher in the model group than in the EA-PC group（ P〈0. 01 ）, while the NBS was significantly lower in the model group than in the EA-PC group （ P〈0. 01 ）. The number of cerebral NgB positive cells in the ischemic penumbra was up-regulated 6 h af- ter CI/RI injury, peaked at 24 h and continued at 72 h. Compared with the sham group, the number of cerebral NgB positive cells of the model group was increased significantly, whereas that of the EA-PC group up-regulated further obviously in comparison with the model group （P〈0.01） . Conclusion EA pretreatment has a significant neuroprotective effect on cerebral ischemia-reper- fusion, which is closely related to its effect in up-regulating NgB protein expression.
出处
《针刺研究》
CAS
CSCD
北大核心
2012年第5期380-384,共5页
Acupuncture Research
基金
国家自然科学基金资助项目(No.81072888
No.30900462)
关键词
脑缺血再灌注
电针预处理
脑红蛋白
神经保护
Cerebral ischemia-reperfusion~ Electroacupuncture preconditioning~ Cerebral neuroglobin~ Neuropro-tection
