摘要
[ Objective] This study aimed to construct the prokaryotic expression vector for Arab/dops/s At4g12500 gene. [ Method] Genomic DNA of wild-type Ar- abidopsis Cold） was extracted as the template for amplification of At4g12.500 gene with PCR technology. The target fragment was double-digested with BamH I and Xho I, and ligated with prokaryotic expression vector pET-28a. [Result] Prokaryotic expression vector pEI28a-At4gI2500 was successfully constructed and trans- formed into Escherichia coli expression strain BL21 （DE3）. [ Conclusion] This study laid the foundation for subsequent expression and functional analysis of At4g12500 gene.
[ Objective] This study aimed to construct the prokaryotic expression vector for Arab/dops/s At4g12500 gene. [ Method] Genomic DNA of wild-type Ar- abidopsis Cold） was extracted as the template for amplification of At4g12.500 gene with PCR technology. The target fragment was double-digested with BamH I and Xho I, and ligated with prokaryotic expression vector pET-28a. [Result] Prokaryotic expression vector pEI28a-At4gI2500 was successfully constructed and trans- formed into Escherichia coli expression strain BL21 （DE3）. [ Conclusion] This study laid the foundation for subsequent expression and functional analysis of At4g12500 gene.
