摘要
为了更准确地鉴定提取鸡骨总RNA的质量,试验分别用核酸蛋白检测仪、1%琼脂糖凝胶电泳和荧光Real-time PCR检测评价3种不同的方法提取成年鸡胸骨总RNA的质量。结果显示,荧光Real-time PCR可更好地鉴定提取的骨总RNA的质量。用核酸蛋白检测仪、1%琼脂糖凝胶电泳能粗略检测RNA的纯度和完整性,但不能反映提取过程中是否引起基因不均一的减少,所检测的纯度也不能精确反映RNA的反转录效率。
In order to accurately analyze the quality and quantity of total RNA from chicken bone, total bone RNA with 3 different extraction methods were detected by Biophotometer plus, 1% agarose gel eleetrophoresis and fluorescent Real-time PCR. The results from Biophotometer plus and 1% agarose gel electrophoresis suggested the three total bone RNA from three different kinds methods could matched with the requirement of experiment. However, the results of real-time PCR showed the total RNA treatment by isopropanol and RNA precipitation solution had excellent quality and quantity. Real-time PCR is a better technique to identify the quality of total bone RNA than Biophotometer plus and 1% agarose ael eleetrophoresis.
出处
《生物学杂志》
CAS
CSCD
2012年第5期92-95,共4页
Journal of Biology
基金
国家自然科学基金(30972234)
教育部博士点基金(200803070021)
江苏省2011普通高校研究生创新计划(CXZZ11-0681)
江苏高校优势学科建设工程资助项目(PAPD)
