摘要
目的建立紫外分光光度法测定枸杞多酚提取物中的总多酚。方法采用紫外分光光度法测定枸杞多酚提取物中总多酚,用水稀释样品,加入显色剂Filin-Ciocalteu试剂,在760 nm处测定吸光度,用同样方法测定并绘制标准曲线。结果紫外分光光度法测定枸杞多酚提取物中总多酚的量分别为24.03%、26.38%、25.45%、28.27%、29.08%,线性范围1～8μg/mL,R2=0.999 5。平均回收率为103.5%,RSD<5%。结论该方法操作简单,准确可靠,适于枸杞多酚提取物中总多酚的测定。
Objective To establish UV-spectrophotometry to determine the content of total polyphenols in polyphenol extract from Lycium barbarum（PELB）.Methods The content of total polyphenols in PELB was detected by using UV-spectrophotometry.The sample was diluted with water,chromogenic agent Filin-Ciocalteu was added,the absorbance was detected at 760 nm by UV-spectrophotometry,and with the same method standard curve was measured and drawn.Results The contents of total polyphenols in different batches of PELB were 24.03%,26.38%,25.45%,28.27%,and 29.08%,respectively,the range of linearity was 1―8 μg/mL,and R2= 0.999 5.The average recovery was 103.5% and the RSD was less than 5%.Conclusion The method is proved to be simple,reliable,accurate,repeatable,and could be used to measure the content of total of polyphenols in PELB.
出处
《药物评价研究》
CAS
2012年第4期273-275,共3页
Drug Evaluation Research
关键词
枸杞多酚提取物
多酚
紫外分光光度计法
polyphenol extract from Lycium barbarum（PELB）； polyphenols； UV-spectrophotometry
