摘要
目的以猫眼草Euphorbia lunulata全草为研究对象,对其有效成分进行分离纯化,并对分离得到的化合物进行活性测定。方法运用硅胶柱色谱、羟丙基葡聚糖凝胶柱色谱、ODS柱色谱以及制备高效液相色谱等方法进行分离,根据理化性质和谱学技术分析确定化合物的化学结构。采用细胞毒活性(MTT)法对提取物、各流分及分离得到的化合物进行抗肿瘤活性评价。结果从猫眼草60%乙醇提取物的醋酸乙酯萃取层中分离得到6个化合物,分别鉴定为大戟素M(1)、泽泻醇A(2)、大戟苷A(3)、甘遂大戟萜酯D(4)、18-羟基泽漆内酯A(5)和β-胡萝卜苷(6)。结论其中化合物1为新化合物,为假白榄烷型骨架二萜类化合物;化合物2～4为首次从该植物中分离得到。经MTT细胞毒活性测试后发现化合物1～4具有较强的肿瘤细胞抑制活性,尤其对人肝癌细胞HepG2有很强的抑制活性。
Objective To study the chemical constituents in the whole plant of Euphorbia lunulata.Antitumor activities of purified compounds were also detected.Methods Compounds were isolated by chromatography on silica gel,hydroxypropyl Sephadex,ODS columns,and preparative HPLC,and their structures were elucidated on the basis of the chemical evidence and spectroscopic analyses.Antitumor activities of the extracts,each fraction,and isolated compounds were evaluated using MTT method.Results Six compounds were obtained from the EtOAc fraction of 60% ethanol extract from the whole plant of E.lunulata and were identified as euphoscopin M（1）,alisol A（2）,euphornin A（3）,kansuiphorin D（4）,18-hydroxyhelioscopinolide A（5）,and β-daucosterol（6）.Conclusion Compound 1 is a new jatrophane-type diterpenoid and compounds 2―4 are reported for the first time from this plant.Compounds 1—4 show the strong inhibitory activities against tumor cells,especially for HepG2 cell.
出处
《中草药》
CAS
CSCD
北大核心
2012年第10期1891-1895,共5页
Chinese Traditional and Herbal Drugs
基金
广东省科技厅科技计划项目(2010A030100018)
国家国际科技合作项目(2011DFA30620)
国家自然科学基金青年基金项目(81001611)
