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Molecular Typing of Leptospira interrogans Strains Isolated from Rattus tanezumi in Guizhou Province,Southwest of China 被引量:13

Molecular Typing of Leptospira interrogans Strains Isolated from Rattus tanezumi in Guizhou Province,Southwest of China
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摘要 Abstract Objective To identify and type three leptospires isolated from Rattus tanezumi in Guizhou Province by using three molecular techniques (PFGE, MLVA, and MLST), reveal the molecular characteristic of causative agents of local leptospirosis and evaluate these three molecular methods based on their detection resolution and efficiency. Methods Three Leptospira strains were isolated from the kidney of Rattus tanezumi and cultured with EMJH medium. PFGE, MLVA, and MLST assays were applied to type the three strains isolated from Rattus tanezumi in Guizhou Province. Results PFGE, MLVA, and MLST typing showed that the three leptospiral isolates matched with leptospiral serogroup Icterohaemorrhagiae serovar Lai. The findings of the genotyping methods were consistent. MLVA and MLST defined genotypes, whereas PFGE allowed the recognition of additional subgroups within the genotypes, and the findings of molecular typing were also consistent with those of traditional techniques. Conclusion Three leptospiral isolates from Guizhou Province matched with leptospiral serogroup Icterohaemorrhagiae serovar Lai, and PFGE, MLVA, and MLST, as reliable molecular techniques for identifying and typing of Leptospira interrogans, would contribute to the active surveillance, outbreak investigation and source tracking for leptospirosis in Guizhou Province. Abstract Objective To identify and type three leptospires isolated from Rattus tanezumi in Guizhou Province by using three molecular techniques (PFGE, MLVA, and MLST), reveal the molecular characteristic of causative agents of local leptospirosis and evaluate these three molecular methods based on their detection resolution and efficiency. Methods Three Leptospira strains were isolated from the kidney of Rattus tanezumi and cultured with EMJH medium. PFGE, MLVA, and MLST assays were applied to type the three strains isolated from Rattus tanezumi in Guizhou Province. Results PFGE, MLVA, and MLST typing showed that the three leptospiral isolates matched with leptospiral serogroup Icterohaemorrhagiae serovar Lai. The findings of the genotyping methods were consistent. MLVA and MLST defined genotypes, whereas PFGE allowed the recognition of additional subgroups within the genotypes, and the findings of molecular typing were also consistent with those of traditional techniques. Conclusion Three leptospiral isolates from Guizhou Province matched with leptospiral serogroup Icterohaemorrhagiae serovar Lai, and PFGE, MLVA, and MLST, as reliable molecular techniques for identifying and typing of Leptospira interrogans, would contribute to the active surveillance, outbreak investigation and source tracking for leptospirosis in Guizhou Province.
出处 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2012年第5期542-548,共7页 生物医学与环境科学(英文版)
基金 supported by the Governor Special Funds for Outstanding Scientific and Technological Talents from Guizhou Province of the People's Republic of China,Guizhou-specifical co-word(2010)90
关键词 L. interrogans PFGE MLVA MLST Rattus tanezumi L. interrogans PFGE MLVA MLST Rattus tanezumi
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  • 1时曼华,屠云人,李庆俊,徐慧栋,于恩庶.我国钩端螺旋体病地理分布的研究[J].中华流行病学杂志,1995,16(5):259-262. 被引量:51
  • 2陈左霞,易槐明,韩小芳,陈恩富.1991~2004年浙江省常山县钩端螺旋体病流行特征分析[J].疾病监测,2006,21(4):192-194. 被引量:1
  • 3全国鼠类专题科研协作组.中国鼠传疾病地理区划[M].中央爱国卫生运动委员会办公室,1984.157.
  • 4陈化新.医学动物分类鉴定[M].中国预防医学科学院流研所,1987..
  • 5Slack AT, Symonds M, Dohnt M, et al. An improved multiple-locus variable number of tandem repeats analysis for Leptospira interrogans serovar Australis : a comparison with fluorescent amplified fragment length polymorphism analysis and its use to redefine the molecular epidemiology of this serovar in Queensland, Australia. J Med Microbiol, 2006, 55 ( Pt 11 ) : 1549-1557.
  • 6Salatin L, Merien F, Gurianova S, et al. Application of multilocus variable-number tandem-repeat analysis for molecular typing of the agent of Leptospirosis. J Clin Microbiol, 2006, 44 ( 11 ) : 3954- 3962.
  • 7Hunter PR, Gaston MA. Numerical index of the discriminatory ability of typing systems : an application of Simpsons's index of diversity. J Clin Microbiol, 1988, 26(11 ): 2465-2466.
  • 8Monot M, Honore N, Gamier T, et al. On the origin of leprosy. Science, 2005, 308(5724) : 1040-1042.
  • 9Matsuoka M, Zhang L, Morris MF, et al. Polymorphism in the rpoT gene in Mycobacterium leprae isolates obtained from Latin American countries and its possible correlation with the spread of leprosy. FEMS Microbiol Lett, 2005, 243(2) : 311-315.
  • 10Majed Z, Bellenger E, Postic D, et al. Identification of variablenumber tandem-repeat loci in Leptospira interrogans sensu stricto. J Clin Microbiol, 2005, 43(2): 539-545.

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