摘要
目的探求L—T4早期治疗妊娠亚临床甲状腺功能减退症（甲减）的效能以及最佳的治疗时间。方法成年Wistar雌鼠被分为6组：对照组（Con组）、临床甲减组（H组）、亚临床甲减组（SCH组）、妊娠第10天开始治疗（GD10组）、妊娠第13天开始治疗（GD13组）以及妊娠第17天开始治疗（GD17组）。水迷宫实验和兴奋性突触后电位检测用于分析各组仔鼠空间学习能力。结果亚甲减组和临床甲减组仔鼠在水迷宫实验中潜伏期时间明显长于对照组以及其电位幅度及斜率增加百分比均明显低于对照组；GD10组和GD13组仔鼠在水迷宫实验中潜伏期时间明显短于亚临床甲减组以及其电位幅度及斜率增加百分比均明显高于亚临床甲减组；L—L补充治疗对于GD17组仔鼠空间学习能力影响较小。结论本研究表明母体亚临床甲减能够导致后代空间学习和记忆能力的损害；应用L—L治疗能够缓解亚临床甲减孕鼠对后代空间学习和记忆能力的损害，妊娠早期是L—L干预治疗的最佳时间。
Objective To investigate the efficacy and optimal time of levothyroxine （ L-T4 ） treatment in pregnant rats with subclinical hypothyroidism. Methods Female adult Wistar rats were divided into six groups （ n = 10 per group） ： control, hypothyroid （H） , subclinical hypothyroid （ SCH）, and SCH treated with L-T4 starting fi＇om the tenth, thirteenth, and seventeenth gestational day （ GD10, GD13 and GD17） , to restore normal thyroid hormone levels. Spatial learning was assessed in progenies by a water maze test and fEPSPs recording. Results Progenies from the SCH and H groups demonstrated significantly longer mean latency in the water maze test and a lower amplification percentage of the fEPSPs＇ amplitude and slope compared with the offspring of the control group. L-T4 treatment in the GD 10 and GD 13 groups significantly shortened mean latency and increased the amplification percentage of the fEPSPs＇ amplitude and slope as compared with the progeny of rats with subclinical hypothyroidism. However, L-T4 treatment in the GDI7 group showed only minimal effect on spatial learning of offspring. Conclusion Maternal subclinical hypothyroidism may impair spatial learning in the offspring; L-T4 treatment started early during pregnancy may alleviate this adverse effect.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2012年第10期845-848,共4页
Chinese Journal of Endocrinology and Metabolism
基金
辽宁省教育厅重点实验室基金（20060905）
