摘要
目的探讨阻断细胞外调节蛋白激酶(ERK)信号转导途径对α-干扰素(IFN-α)Janus激酶-信号转导和转录激活子(JAK-STAT)信号转导途径分子和抗病毒蛋白表达的影响。方法以人肝胚瘤细胞株HepG2细胞为研究对象,以单独IFN-α及IFN-α和ERK抑制剂PD98059联合处理细胞,分析阻断ERK途径对HepG2细胞IFN-α应答的变化。结果反转录PCR(RT-PCR)结果显示,HepG2细胞经处理后,细胞内抗病毒蛋白和JAK-STAT信号转导途径分子STATl、STAT2、IRF9 mRNA表达均增高,其中联合处理的表达升高更加明显。Western blot分析STAT1、STAT2结果与RT-PCR结果一致,另外,细胞经IFN处理后JAK-STAT信号转导途径抑制因子3(SOCS3)的表达升高,联合处理后,SOCS3表达减少。结论在体外细胞模型中,ERK信号转导途径可能通过调节SOCS3蛋白的表达而参与IFN-α抗病毒效应。
Objective To investigate the influence of blocking the signal transduction pathway of ERK on IFN-in- duced antiviral proteins in HepG2 cells. Methods HepG2 cells were treated with IFN-α and ERK inhibitor PD98059, and the mRNA levels of MxA,2＇-5＇OAS, PKR, STAT1, STAT2, IRF9 and SOCS3 were assessed by RT-PCR after being treated with IFN-ot and PD98059. Meanwhile, the expressions of STAT1, STAT2 and SOCS3 pro- teins were detected by Western blot. Results After ERK signaling pathway of HepG2 cells was pre-blocked, the ex- pression levels of STAT1, STAT2 and MxA, 2＇-5＇OAS, PKR, STAT1, STAT2, IRF9 mRNA were enhanced, howev- er, SOCS3 and its mRNA were decreased. Conclusion ERK signal pathway is involved in the antiviral activity of IFN-α through decreasing the expression of SOCS3 in HepG2 cells.
出处
《安徽医科大学学报》
CAS
北大核心
2012年第11期1287-1290,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81171662)
安徽省卫生厅科学基金(编号:2010C057)
