期刊文献+

五种结核病实验室检测方法对结核病诊断价值的效果评价 被引量:4

Clinical evaluation on the detection of tuberculosis by five methods
下载PDF
导出
摘要 目的评价涂片法、BACTECMGIT960快速培养法、改良罗氏培养法、荧光定量PCR法和斑点免疫层析法在结核病实验室快速诊断方法中的作用和地位。方法对1260例结核病患者(结核病组)和100例非结核病患者(非结核病组)的各类标本采用涂片法、快速培养法、改良罗氏培养法、荧光定量PCR法进行检测,同时分离患者外周血血清进行结核抗体检测,并对这五种检测方法的结果进行分析比较。结果五种实验室检测方法对结核病组阳性标本和非结核病组阴性标本的检测结果差异均有统计学意义(χ2=466.31,χ2=216.14,P均〈0.05)。对于结核病组的涂阳和涂阴标本,快速培养法和荧光定量PCR法检测的灵敏度及准确度均高于其他三种方法,而涂片法、改良罗氏培养法、快速培养法的特异性均为100.0%,高于荧光定量PCR法和斑点免疫层析法;快速培养法的阳性检出率均较改良罗氏培养法高,差异均有统计学意义(χ2=3387.00,χ2=4233.00,P均〈0.05),平均培养时间也较改良罗氏培养法短;但快速培养法与荧光定量PCR法的阳性检出率差异均无统计学意义(P均〉0.05)。结论BACTECMGIT960快速培养法和荧光定量PCR法是诊断结核病快速、有效的检测方法,其阳性检出率高,且能缩短培养时间,BACTECMGIT960快速检测系统还能进行快速药物敏感性试验,为结核病的快速诊断提供依据。 Objective To evaluate the diagnosis value of smear method, BACTEC MGIT 960 rapid culture method, improved roche culture method, fluorescent quantitative PCR method and dot immunochro- matographic assay to detected tuberculosis. Methods The clinical specimens of 1260 patients with tuberculo- sis(tuberculosis group) and lO0 patients with non-tuberculosis (non-tuberculosis group) were detected by smear method, rapid culture method, improved roche culture method, fluorescent quantitative PCR method, and the serum specimens of patients were detected by dot immunochromatographic assay. The results were ana- lyzed. Results There were statistically significant differences of five methods in the positive detection rates of tuberculosis group and negative detecting rates of non-tuberculosis group (χ2= 466.31 ,χ2= 216.14, Pall〈 0.05 ). To the smear-positive specimens and smear-negatives pecimens of tuberculosis group, the sensitivity and ac- curacy of rapid method and fluorescent quantitative were all higher than other methods, but the specificity (100.0%) of rapid method, improved roche culture method and smear method were all 100.0%, which were higher than fluorescent quantitative PCR method and dot immunoehromatographie assay. The positive detect rates of rapid culture method were all higher than improved roche culture method, the differences had statisti- cal significance (χ2= 3387.00 ,)(χ2= 4233.00,Pa11〈 0.05 ). And the average culture time of rapid culture method were shorter than improved roche euhure method. There were no statistically significant differences between rapid culture method and fluorescent quantitative PCR method in positive detection rates (Pall〉 0.05 ). Con- clusion BACTEC MGIT 960 rapid culture method and fluorescent quantitative PCR method were fast and ef- fective detection methods. They had a high postive deetection rates, and could shorten culture time, BACTEC MGIT 960 rapid detection system also could make rapid drug sensitivity test, and provide the basis for the rapid diagnosis of tuberculosis.
出处 《实用检验医师杂志》 2012年第3期157-160,共4页 Chinese Journal of Clinical Pathologist
基金 石家庄市科技局攻关项目(111146643)
关键词 结核病 BACTEC MGIT 960 荧光定量PCR 斑点免疫层析法 诊断 Tuberculosis BACTEC MGIT 960 Fluorescent quantitative PCR Dot immunochro-matographie assay Diagnosis
  • 相关文献

参考文献5

二级参考文献31

  • 1谢强,孙卫红,翁丽珍,邹盛华.BACTEC MGIT-960临床应用与结果分析[J].临床肺科杂志,2004,9(4):350-352. 被引量:6
  • 2曹晓慧,张媛媛,万康林.PCR技术在结核病诊断中的应用[J].中国人兽共患病杂志,2005,21(12):1110-1113. 被引量:10
  • 3结核病诊断细菌学检验规程[J].中国防痨杂志,1996,18(1):28-31. 被引量:799
  • 4World Health Organization. Global tuberculosis control: surveillance, planning, finances [R]. WHO Report, 2006.
  • 5Mghani B, Stutman H R. Polymerase chain reaction for diagnosis of M tuberculosis: comparison of simple boiling an d a conventional method for DNA extraction [ J ]. Biochem Mol M ed, 1996,57 ( 1 ) : 14-18.
  • 6Rees C B, Li W. Development and application of fl real-time quantitative PCR assay for determining CYP1A transcripts in three genera of salmonids [ J ]. Aquat Toxicol, 2004,66(4) :357-368.
  • 7Rondini S, Mensah-Quainoo E, Troll H, et al. Development and application of real-time PCR assay for quantification of Mycobacterium ulcerans DNA [J]. J Clin Microbiol, 2003,41 (9): 4231-4237.
  • 8Heid C A, Stevens J, Livak K J, et al. Real time quantitative PCR [J]. Genome Res, 1996,6(10) :986-994.
  • 9Fend R, Geddes R, Lesellier S. Use of an electronic nose to diagnose Mycobacterium boris infection in badgers and cattle [J]. J Clin Microbiol, 2005,43(4) : 1745-1751.
  • 10Pas S D, Fries E, De Man R A, et al. Development of fl quantitative real-time detection assay for hepatitis B virus DNA and comparison with two commercial assays [J]. J Clin Microbiol, 2000,38 (8):2897-2901.

共引文献2911

同被引文献74

  • 1张阳,韦红.三种方法检测结核分枝杆菌的应用评价[J].河南预防医学杂志,2021,32(9):719-720. 被引量:4
  • 2张毅军,毛晓辉,白永辉.痰噬菌体联合PPD、血清抗结核抗体检测对肺结核的诊断价值[J].陕西医学杂志,2007,36(7):868-869. 被引量:1
  • 3中国疾病预防控制中心结核病预防控制中心.全国第五次结核病流行病学抽样调查结果简介.中国结核病预防控制,2011,3:14-16.
  • 4Miller K, Harrington SM, Procop GW. Acid-fast Smear and Histopathology Results Provide Guidance for the Appropriate Use of Broad-Range Polymerase Chain Reaction and Sequencing for My- cobacteria. Arch Pathol Lab Med, 2015,139:1020-1023.
  • 5Seo AN, Park HJ, Lee HS, et al. Performance Characteristics of Nested Polymerase Chain Reaction vs Real-Time Polymerase Chain Reaction Methods for Detecting Mycobacterium tuberculosis Complex in Paraffin-Embedded Human Tissues. Am J Clin Pathol, 2014,142:384-390.
  • 6Lee HS, Park KU, Park JO, et al. Rapid, Rapid, sensitive, and spe- cific detection of Mycobacterium tuberculosis complex by real-time PCR on paraffin-embedded human tissues. J Mol Diagn,2011,13: 390-394.
  • 7Luo RF, Scahill MD, Banaei N. Comparison of single-copy and mul- ticopy real-time PCR targets for detection of Mycobacterium tubercu- losis in paraffin-embedded tissue. J Clin Microbiol,2010,48:2569- 2570.
  • 8World Health Organization. Global tuberculosis report 2015 [ R]. Geneva:World Health Organization, 2015.
  • 9赵雁林,刘志敏.结核病诊断实验室检验规程[M].北京:中国教育文化出版社,2006:173-177.
  • 10王宇.2010年全国第五次结核病流行病学抽样调查报告[M].北京:军事医学科学出版社,2011:7-50.

引证文献4

二级引证文献36

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部