摘要
目的:探讨OPN(osteopontin)、黏附分子CD44拼接变异体v6(CD44v6)和基质金属蛋白酶-9(MMP-9)在喉鳞状细胞癌(LSCC)组织中的表达及临床意义。方法:采用免疫组织化学法检测47例LSCC及10例癌旁正常喉咽黏膜中OPN、CD44v6和MMP-9的表达,并分析三者与LSCC临床病理特征的关系及三者表达的相关性。结果:①OPN、CD44v6和MMP-9蛋白的阳性表达率在LSCC组织中分别为63.8%、76.6%和68.1%,在癌旁正常组织中分别为10%、30%和0;2组3种蛋白表达阳性率比较均差异有统计学意义(均P<0.01);②OPN和CD44v6的表达与LSCC临床分期、病理分级、淋巴结转移有关(均P<0.05),MMP-9与LSCC临床分期、淋巴结转移有关(P<0.05),OPN、CD44v6和MMP-9的阳性表达与患者年龄、性别、肿瘤位置无关(P>0.05);③LSCC组织中OPN与CD44v6、MMP-9的表达均呈正相关(r=0.421,P<0.01;r=0.340,P<0.05),CD44v6与MMP-9的表达呈正相关(r=0.376,P<0.01)。结论:OPN、CD44v6和MMP-9在LSCC中呈高表达,它们之间的表达呈正相关性,三者可能共同参与了LSCC的侵袭和转移过程。
Objective:To investigate the expression and clinical significance of OPN,CD44v6 and MMP-9 in laryngeal squamous cell carcinomas(LSCC).Method:The expression of OPN,CD44v6 and MMP-9 in the 47 cases of LSCC and 10 cases normal mucosa were detected by immunohistochemical method.The correlation between OPN,CD44v6 and MMP-9 proteins expressions and the correlation between them and clinicopathological feature were also studied.Result:①The positive expression rates of OPN,CD44v6 and MMP-9 were 63.8%,76.6% and 68.1% in LSCC of 47 cases and,and 10%,30% and 0 in the tumor adjacent issue.②The positive rate of OPN and CD44v6 was significantly correlated with the clinical staging,the pathological grading and the metastasis of cervical lymph node(P<0.05).The positive rate of MMP-9 was correlated with the clinical staging and the metastasis of cervical lymph node(P<0.05).The positive rates of OPN,CD44v6 and MMP-9 were not correlated with patient's age,sex and tumor location(P>0.05).③The expression of OPN was well correlated with the positive rate of CD44v6 and MMP-9 in LSCC(r=0.421,P<0.01;r=0.340,P<0.05).The expression of positive rate of CD44v6 and MMP-9 was well related in LSCC as well(r=0.376,P<0.01).Conclusion:There is a high level of expression of OPN,CD44v6 and MMP-9 in LSCC.OPN,CD44v6 and MMP-9 are positively related and involved in the invasion and metastasis of LSCC.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2012年第21期989-992,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
