摘要
内质网型ω-3脂肪酸去饱和酶(FAD3)是不饱和脂肪酸合成途径中催化十八碳二烯酸[18∶2(9,12)]转化为十八碳三烯酸[18∶3(9,12,15)]的关键酶。通过RT-PCR的方法从番茄叶片克隆到LeFAD3的全长cDNA,基因全长为1 184bp,开放阅读框(ORF)为1 134bp,注册号为EU251190。将该基因正反向序列分别插入带有35S-CaMV启动子pBI121表达载体下游,获得正、反义表达载体,转化农杆菌LBA4404,利用农杆菌介导法转化番茄,获得转基因番茄植株。
The endoplasmic reticulum omega-3 fatty acid desaturase（FAD3） is the key enzyme which catalyzes 18∶2（9,12） to form 18∶3（9,12,15） in the unsaturated fatty acid biosynthesis.The full length of the cDNA of LeFAD3 was isolated by RT-PCR from tomato leaves,which contains of 1 184 bp nucleotides and a 1 134 bp open reading frame（ORF） of 1 134 and accession number EU25119.The full-length sequences of sense-and antisense-LeFAD3 were inserted into pBI121 vector downstream of the 35S-CaMV promoter to form sense constructs and antisense constructs.The 35S-CaMV LeFAD3 constructs were first introduced into Agrobacterium tumefaciens LBA4404.Leaf disk infected with A.tumefaciens transformation was used to transform tomato.The transgenic plants of tomato were obtained.
出处
《西北农业学报》
CAS
CSCD
北大核心
2012年第9期90-94,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
国家自然科学基金(31101554)
浙江省自然科学基金(Y3110340)
浙江农林大学启动基金(2351001059
2351000812)
关键词
番茄
内质网型ω-3脂肪酸去饱和酶
三烯脂肪酸
表达载体
Tomato（Lycopersicon esculentum）
Endoplasmic reticulum-localized omega-3 fatty acid desaturase
Trienoic fatty acids
Expression vector
