摘要
目的:探讨甲基莲心碱(neferine)对氧化损伤的大鼠乳鼠心肌细胞的保护作用。方法:分离培养SD乳鼠心肌细胞,建立心肌细胞过氧化损伤模型,将细胞分为7组,即空白对照组(control)、溶剂对照组(DMSO)、氧化损伤组(H2O2)、氧化损伤加入槲皮素对照组(quercetin+H2O2)、氧化损伤加入莲心碱低、中、高浓度组(甲基莲心碱+H2O2、甲基莲心碱-M+H2O2、甲基莲心碱-H+H2O2)。将300μmol/LH2O2作用于加入槲皮素及不同浓度甲基莲心碱预培2 h的心肌细胞,继续培养24h,以细胞毒四唑盐(MTT)比色试验,乳酸脱氢酶(LDH)、谷胱甘肽过氧化物酶(GSH-px)、丙二醛(MDA)、流式细胞凋亡率、Caspase酶活性为检测指标。结果:甲基莲心碱呈剂量依赖性降低H2O2对心肌细胞活力的影响,降低MDA、LDH量,增加细胞GSH-px活性,并显著抑制Caspase-3和9活性,减少细胞凋亡数量,各指标差异具有统计学意义(P<0.01)。结论:甲基莲心碱可保护和修复过氧化氢诱导的心肌细胞的损伤,其作用可能是通过抗氧化、增加细胞GSH-px活性,进而抑制Caspase-3和9活性,减少细胞凋亡。
Objective ：To study the effect of neferine on cardiomyocyte cells injuries induced by H2 02. Methods：Endo- thelial cells were cultured in vitro and divided into seven groups：control group, solvent group, H2 02 group, quercetin plus H202 group, neferine - L, M, H plus H2 02 groups respectively. Cardiomyocyte cells were incubated with 300 .9 mol/L H202 for 24 hours in the absence or presence of various concentrations of neferine and quereetin. Then the apoptotie inde- xes were detected by flow eytometrie. Meanwhile, the amount of Malondialdehyde （ MDA）, the activty of GSH - px and caspase were measured. Results：Incubation of eardiomyoeyte cells with H2 02 for 24h decreased the rate of apoptotie cells, the release of LDH,inhibited the activity of caspase - 3 and 9, and reduced the contents of MDA, accompanied by the de- crease in GSH -px activity. Pretreatment of neferine with 1 ～ 30μmol decreased these H202 -induced changes in a con- centration - dependent manner（ P 〈 0.01 ） . Conclusion ： Neferine posses a protective effect against eardiomyocyte cells in- juries induced by H2 02, which is probably via anti - oxidation, increasing the activity of GSH - px, reducing caspase - 3 and 9 activity and inhibiting the apoptosis of endothelial cells.
出处
《中华中医药学刊》
CAS
2012年第10期2310-2312,共3页
Chinese Archives of Traditional Chinese Medicine
关键词
H2O2
莲心碱
心肌细胞
凋亡
hydrogen peroxide
neferine
cardiomyocyte cells
cells cultured
apoptosis
