摘要
目的:探讨抑郁症患者外周血脑源性神经营养因子(BDNF)蛋白和mRNA表达水平与自杀行为、抑郁症状的关联。方法:共纳入符合中国精神障碍分类与诊断标准第3版诊断标准的抑郁症患者65例,按有无自杀未遂行为分为自杀未遂组(n=31)、无自杀行为组(n=34),健康对照30名。采用汉密顿抑郁量表(HAMD)、Beck绝望量表(BHS)、自杀意念自评量表(SIOSS)评估病例组的抑郁、绝望与自杀意念;采用酶联免疫吸附法测定血清BDNF蛋白浓度,实时荧光定量聚合酶链式反应(PCR)法测定BDNF mRNA相对表达量。结果:自杀未遂组与无自杀行为组的BDNF蛋白浓度[(57.3±9.2)ng/mL,(76.0±25.7)ng/mL vs.(113.8±44.4)ng/mL,均P<0.05]与BDNF mRNA相对表达量[(0.2±0.0),(0.5±0.0)vs.(1.0±0.1),均P<0.05]均低于正常对照组,自杀未遂组的血清BDNF蛋白浓度和mRNA相对表达量低于无自杀组(P<0.05)。病例组(n=65)血清BDNF蛋白浓度与SIOSS总分(r=-0.24,P<0.01)呈负相关,与BDNF mRNA相对表达量(r=0.28,P<0.01)呈正相关;BDNF mR-NA相对表达量与HAM D总分(r=-0.46,P<0.01)、BHS总分(r=-0.42,P<0.01)、SIOSS总分(r=-0.43,P<0.01)呈负相关,与病程(r=0.19,P<0.05)呈正相关。结论:研究提示外周血BDNF蛋白和mRNA水平低可能是抑郁发作患者出现自杀行为的危险因素之一。
Objective： To explore the association of brain-derived neurotrophic factor （BDNF） protein in pe- ripheral blood and mRNA expression to suicidal behavior in patients with depression. Methods： Sixty-five patients with depression meeting the Chinese Classification and Diagnostic Criteria for＇ Mental Disorders, Third Edition （ CC- MD-3） criteria and 30 healthy controls were selected. The patients were divided into group with suicide attempts （ n = 31） and group without suicide attempts （n = 34）. The patients were assessed with the Hamilton Depression Scale （HAMD）, Beck Hopelessness Scale （BHS）, and Self-rating Idea of Suicide Scale （SIOSS） to evaluate the severity of depression symptoms, hopelessness, and suicide idea. In all subjects, the BDNF mRNA expression was measured using real-time quantitative polymerase chain reaction （PCR）, and the serum level of BDNF was detected with ELISA method. Results： BDNF protein [ （57.3 ± 9. 2） ng/mL, （76. 0 ± 25.7） ng/mL vs. （ 113.8 ± 44.4） ng/mL, Ps 〈 0.05 ] and mRNA expression [ （0. 2± 0. 0）, （0. 5 ± 0. 0） vs. （ 1.0± 0. 1）, Ps 〈 0. 05 ] were both lower in pa- tients with suicide attempts and patients without suicide attempts than in control group. Patients with suicide at- tempts had lower BDNF expression than those without suicide attempts （Ps 〈 0. 05）. In patients with depression （n =65）, the serum BDNF protein level was negatively correlated with SIOSS score （r = -0. 24, P 〈0. 01）, and was positively correlated with BDNF mRNA expression （ r = 0. 28, P 〈 0. 01 ）. The expression level of BDNF mRNA was negatively correlated with scores of HAMD （ r = - 0. 46, P 〈 0. 01 ）, BHS （ r = - 0.42, P 〈 0. 01 ） and SIOSS （r = -0.43, P 〈0. 01）, and was positively correlated to disease course （r =0. 19, P 〈0. 05）. Conclusion： The re- suits suggest that the decreased levels of brain-derived neurotrophic factor （BDNF） protein in peripheral blood and gene expression may be related to the suicidal behavior in patients with depression.
出处
《中国心理卫生杂志》
CSSCI
CSCD
北大核心
2012年第10期726-730,共5页
Chinese Mental Health Journal
基金
河南省卫生科技创新型人才工程专项经费资助(4173)
关键词
抑郁症
自杀行为
脑源性神经营养因子
depression
suicidal behavior
brain-derived neurotroohic factor
