摘要
目的:探讨《中国药典》胰岛素生物测定的简化方法及其快速检测。方法:按照胰岛素生物检定方法,但动物数减半、随机设计,并使用血糖仪快速测定小鼠血糖值,考察多批胰岛素生物活性,与原有胰岛素生物检定方法及血糖测定方法对比。结果:简化的胰岛素生物检定方法具有特异性、重复性,可半定量测定胰岛素生物活性。应用血糖仪快速测定小鼠血糖值具有特异性、线性关系,精密度高,重复性好,与全自动生化仪测定结果基本相近,其差异没有显著性(P>0.05),并且高度相关(r=0.994 9,P<0.01)。结论:胰岛素生物测定的简化及快速实验方法是可行的。
Objective：To investigate the simplified bioassay method for insulin in Chinese Pharmacopoeia and its rapid detection. Method ： The several batches of insulin biological activity were determined by the insulin bioassay method of half the number of animals and randomized design, and by a blood glucose meter for rapidly determination of blood glucose values. Results ： The simplified insulin bioassay method with specificity and repeatability can be used for semi-quantitative determination of insulin biological activity. The blood glucose levels in mice which were rapidly measured by blood glucose meter with specific, linear relationship, precision, repeatability, were similar with the results by automatic biochemical analyzer. Their differences were not significant （ P 〉 0.05 ） and they are highly correlated（ r = 0. 994 9, P 〈 0. 01 ） Conclusions：The simplify rapid method for insulin bioassay is feasible.
出处
《中国药品标准》
CAS
2012年第5期336-340,共5页
Drug Standards of China
关键词
中国药典
胰岛素
简化快速生物测定方法
血糖仪
Chinese Pharmacopoeia
insulin
simplified experimental bioassay method
blood glucose meter
