摘要
目的探讨人工培育与自然感染的日本血吸虫感染性钉螺对小鼠感染性的差异。方法将120只昆明种小鼠随机分为2组,将人工与自然感染性钉螺用常规法逸蚴,每只小鼠感染40条尾蚴,以平均虫荷、成虫发育率、每克肝脏虫卵数(肝卵EPG)和每克粪便虫卵数(粪卵EPG)为指标进行观察,并对观察结果进行统计分析。结果自然感染性钉螺感染小鼠的平均虫荷、成虫发育率、肝卵EPG及粪卵EPG分别为27.43±3.78、68.53±9.44、19 800.97±6 752.59、196.37±11.56,均明显高于人工组的23.93±4.93、59.83±12.32、5 803.69±1 560.49、107.73±10.32(P均<0.05);相同数量的尾蚴感染后,雄性组小鼠的虫荷、发育率、肝卵EPG均高于雌性组(P均<0.05)。结论自然感染性钉螺对小鼠的感染性明显高于人工感染性钉螺,且雄性小鼠比雌性小鼠更易感染血吸虫尾蚴。
Objective To explore the infectivity difference between the mice challenged by laboratory？cultivated and field collected Schistosoma japonicuminfected Oncomelania hupensis snails. Methods A total of 120 Kunming mice were randomly divided into two groups. S. japonicuminfected O. hupensis releasing cercariae was according to routine cercaria shedding method. Each mouse was challenged by 40 cercariae. The wormload,the development rate,EPG of liver and EPG of feces in the mice were calculated. Results The mean wormload,adult worm development rate,EPG of liver and EPG of feces in the group of mice infected by field collected S. japonicum？infected snails were 27.43±3.78,68.53±9.44,19 800.97±6 752.59 and 196.37±11.56,respectively,which were significantly higher than those in the group challenged by cercariae from laboratorycultivated S. japonicuminfected snails（23.93±4.93,59.83±12.32,5 803.69±1 560.49 and 107.73±10.32）（P 〈 0.05）. The mean wormload,adult worm development rate and EPG of liver in the male mouse group were higher than those in the female mouse group（P 〈 0.05）. Conclusion The cercariae released from field collected S. japonicuminfected snails have more aggressive infection ability,compared with the cercariae released from the laboratorycultivated S. japonicumnfected snails and the results also show male mice are more susceptibility to the schistosome infection than female mice.
出处
《中国血吸虫病防治杂志》
CAS
CSCD
2012年第5期579-580,584,共3页
Chinese Journal of Schistosomiasis Control
基金
“十一五”国家科技支撑计划资助(2009BAI78B07)
湖南省卫生厅科研基金项目(132008-058)
关键词
日本血吸虫
钉螺
尾蚴
感染性
小鼠
Oncomelania hupensis
Schistosome
Cercaria
Infectivity, Mouse
