摘要
‘紫红1号’是新疆红肉野苹果[Malussieversiif.neidzwetzkyana(Dieck)Langenf.]与‘富士’苹果杂交的F1代中的一株果肉全红的株系。以其为试材,测定其果肉的花色苷、多酚、类黄酮含量及其抗氧化能力,结果表明其果肉花色苷含量为228.3mg·kg-1FW,总酚含量为2523mg·kg-1FW,类黄酮含量为2514mg·kg-1FW,其抗氧化能力(ferric reducing antioxidant power,FRAP)为13.39μmol·g-1。利用液质联用(UPLC-PAD-/MS/MS)鉴定了红肉苹果果肉花色苷的主要组分有9种,其中4种分别为矢车菊3–O–葡萄糖苷、矢车菊3–O–半乳糖苷、矢车菊3–O–木糖苷、矢车菊3–O–阿拉伯糖苷,其中矢车菊3–O–半乳糖苷占73.37%,另外5种暂不能确定其结构。本研究表明花色苷的稳定性,发现该色素在pH1～3,40℃以下比较稳定,高温加速花色苷的降解。花色苷对光照敏感,暴露在室内自然光下15d其残留率为41.53%。
‘Zihong 1’apple is red-flesh apple,which belonged to F 1 of Xinjiang wild red-flesh apple[Malus sieversii f. neidzwetzkyana（Dieck）Langenf.] and‘Fuji’apple. Pulp of‘Zihong 1’apple was taken as material to determine the anthocyanin content,total phenolic content,flavonoid content and antioxidant activity. The results showed that they were 228.3 mg·kg-1 FW,2 523 mg·kg-1 FW,2 514 mg·kg-1 FW and 13.39 μmol·g-1 ,respectively. Nine anthocyanins were found in red pulp of‘Zihong 1’ apple assayed by UPLC-PAD-/MS/MS,of which four kinds of anthoyanins were detected as cyanidin 3-O-galactoside,cyanidin 3-O-glucoside,cyaniding 3-O-arabinoside and cyanidin 3-O-xyloside. cyanidin 3-O-galactoside was the main anthcyanin,which accounted for 73.3% of total anthocyanins. Structure of the other five anthocyanins couldn’t be determined temporarily. The stability of the anthocyanins was studied and found that the anthocyanins were more stable in aqueous solution which pH value was between 1 and 3,below 40 ℃. The high temperature accelerated degradation of the anthocyanins. The anthocyanins was sensitive to light and the residual amount was only 41.53%.
出处
《园艺学报》
CAS
CSCD
北大核心
2012年第10期1991-1998,共8页
Acta Horticulturae Sinica
基金
国家‘863’计划重点项目(2006AA100108)
国家自然科学基金项目(30871679)
山东省农业良种工程项目
关键词
苹果
红肉
花色苷
多酚
抗氧化
花色苷稳定性
apple
red-flesh
anthocyanin
phenolic
antioxidant
anthocyanins stablity
