摘要
A phenoxazinium-based chemosensor （1） bearing di（2-picolyl）amine unit was successfully synthesized. The result shows that it is a red-emitting and full water-soluble chemosensor for the selective detection of Cu2＋ in pure water. The fluorescence on-off mechanism was studied by ab initio calculations. To confirm the suitability of 1 for biological applications, it was employed in the fluorescence detection of the intracellular Cu2＋ with cultured KB cells. The results indicated that 1 had good membrane permeability and could be useful for the fluorescence micro- scopic imaging.
A phenoxazinium-based chemosensor （1） bearing di（2-picolyl）amine unit was successfully synthesized. The result shows that it is a red-emitting and full water-soluble chemosensor for the selective detection of Cu2＋ in pure water. The fluorescence on-off mechanism was studied by ab initio calculations. To confirm the suitability of 1 for biological applications, it was employed in the fluorescence detection of the intracellular Cu2＋ with cultured KB cells. The results indicated that 1 had good membrane permeability and could be useful for the fluorescence micro- scopic imaging.
基金
We are grateful to Professor Masataka Ihara （Hoshi University, Japan） for the helpful discussions regarding the synthesis of 1. This work was financially supported by the National High Technology Research and Development Program of China （863 Program, SQ2009AA06XK1482331）, the National Environmental Community Project of China Natural Science Foundation （200909044）, the National of China （51273136） and the Project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
