摘要
目的 探讨铝对人胚大脑神经细胞的毒作用及其机制。方法 采用无血清体外细胞培养方法 ,观察铝对人胚大脑神经细胞的生长发育及功能的影响 ,同时进行光镜和电镜形态学观察。结果 高铝组的细胞存活数低于对照组 ,差异有显著性 ,光镜观察在高铝组可见大量变性坏死细胞 ;随铝剂量的增加神经细胞的DNA含量、神经细胞特异性烯醇化酶、胆碱酯酶活性也随之下降 ,呈负相关关系 ,并且低铝组、高铝组低于对照组 ,差异有显著性。神经细胞过氧化脂质含量 ,高铝组高于对照组 ,差异有显著性 ,并与铝剂量呈正相关关系。高剂量铝引起大脑神经细胞超微结构的损害表现为各种膜结构的损害及神经微丝微管排列紊乱。结论 铝可抑制大脑神经细胞的分化成熟及功能 ,并随铝剂量的增加而增强 ,其毒作用机制可能是通过抑制神经细胞抗氧化能力产生脂质过氧化而导致细胞各种膜结构的损害。
Objective To study the toxic effect of aluminum on human embryonic cerebral neurocytes in vitro studies. Methods Human embryonic cerebral neurocytes were divided into three groups (control,low Al and high Al groups). The growth, development and morphology of neurocytes were observed by light and electron microscopy. The protein, DNA,lipid peroxide (LPO) contents and the activity of neuron specific enolase (NSE) and cholinesterase (CHE) of neurocytes were detected. Results The number of viable cells in high Al group was significantly lower than that in the control group ( P< 0 05). The number of swelling, degenerated and necrotic cells was significantly increased in high Al group. The DNA content and the activity of NSE and CHE of neurocytes were significantly decreased with the increase of Al concentrations ( P< 0 05). The LPO content of neurocytes in the high Al group was significantly higher than that in the control group ( P< 0 05). There was a positive correlation between the Al dose and LPO content of neurocytes ( P< 0 05). The electron microscopy study showed that the membrane structure of cell was damaged in the high Al group and the arrangement of microtubules in the processes was disordered. Conclusions The growth, development and function of human embryonic cerebral neurocytes was inhibited in the high Al group. The neurotoxicity of Al may be caused by lipid peroxidation and the damage of cell membrane.
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2000年第2期106-108,共3页
Chinese Journal of Preventive Medicine
基金
天津市 2 1世纪青年科学基金! ( 95 3 60 8911)
关键词
脑
神经元
细胞培养
铝中毒
Aluminum
Brain
Neurons
Cells cultured
