摘要
目的 :观察纳洛酮和丹参合用处理兔感染性休克模型后的循环、代谢及肺标本的光镜和电镜下的病理改变。方法 :选择 30只健康家兔 ,随机分为五组 ,通过结扎盲肠末端并造成穿孔 ,复制与临床类似的家兔感染性休克模型后 ,给予相应的治疗。 4小时后经颈总动脉置管测MAP ,并抽血测定乳酸盐、丙二醛及动脉血气 ;18小时后活杀动物取肺标本。结果 :纳洛酮组、纳洛酮丹参合用组可明显地升高MAP ,并可明显降低休克模型的血乳酸盐、花生四烯酸的代谢产物丙二醛的水平。治疗组的肺水含量明显低于对照组。光镜见纳洛酮丹参合用组的肺淤血较轻 ,炎细胞浸润亦明显轻于对照组及纳洛酮组。电镜观察见该组的线粒体膜仍保持完好 ,未见破裂或空泡化 ;而对照组及纳洛酮组的线粒体膜则有明显的断裂、解体及线粒体的空泡化。结论 :纳洛酮或纳洛酮丹参合用均可明显改善感染性休克的代谢变化 ,保护脏器功能。因此纳洛酮和丹参合用在改善肺组织的病理形态学方面远较单纯纳洛酮为好 。
Objective:Toobservethechangesincirculation ,metabolismandpathologyofthelungpreparationinsepticshockrabbitstreatedwithnaloxoneplusdansheng .Methods:30rabbitswererandomlydevidedintofivegroupsandseptic shockmodelwasmadebysurgicalligationandperforationofthecaecumend .Theanimalsweretreatedwithdifferentdrugs andplaceboincontrolgroup .MAP ,bloodlactate ,MDAandbloodgasweremeasuredat 4hafterandthelungpreparationwastakenbykillingtheanimalat 18hafterthetreatment.Results:MAPwasincreasedsignificantlyandbloodlactateandMDA weredecreasedmarkedlyintheanimalstreatedwithnaloxoneplusdansheng .Lungwaterwasremarkablylowerinthetreated groupsthanthatincontrolgroupandmitochondriamambranewaskeptcompleteobservedunderelectronmicroscopeinthetreatedgroups.Conclusion :Acombinationofnaloxonewithdanshengcanimprovethechangesincirculationandmetabolism ofthesepticshockrabbits,whichisbetterthanthatofthesepticshockedanimaltreatedwithnaloxonealone .
出处
《临床麻醉学杂志》
CAS
CSCD
2000年第3期133-135,共3页
Journal of Clinical Anesthesiology
