摘要
目的采用HPLC法建立萹蓄药材的指纹图谱。方法采用CAPCELL PAK C18MG(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸溶液(B)为流动相,线性梯度洗脱;检测波长340 nm;柱温30℃;流速1.0 mL min-1。结果构建的指纹图谱有18个共有峰,确认了其中8个峰的成分分别为绿原酸、杨梅苷、金丝桃苷、木犀草苷、扁蓄苷、槲皮苷、胡桃宁、木犀草素。结论本研究为萹蓄药材的质量控制提供了新方法。
Objective To establish an HPLC f ingerprint of Polygonum aviculare L..Methods HPLC fingerprint was established with CAPCELL PAK C18 MG（250 mm×4.6 mm,5 μm） column.Acetonitrile（A） and 0.1% phosphoric acid（B） were used as the mobile phase at 1.0 mL · min-1.The detection wavelength and column temperature were 340 nm and 30 ℃respectively.Results In this fingerprint,18 characteristic peaks were selected as the fingerprint peaks.Eight of these characteristic peaks were identified as chlorogenic acid,myricitrin,hyperoside,luteoloside,avicularin,quercitrin,juglanin,luteolin.Conclusion The method can be used for the quality control of Polygonum aviculare L..
出处
《中南药学》
CAS
2012年第10期773-775,共3页
Central South Pharmacy
基金
广东省科技计划资助项目(编号:2010B050100013)
