摘要
目的利用重组白念殊菌果糖二磷酸醛缩酶（fructose-bisphosphatealdolase，Fbal）抗原，建立测定人血清中抗白念珠菌FbalIgG抗体的ELlSA法，评估其在侵袭性念珠菌病（invasivecandidiasis，IC）诊断中的应用价值。方法收集经血培养确诊的各类IC患者（110例）、念珠菌定植者（50例）、细菌感染者（147例）及健康体检者（200例）血清，用重组Fbal包被ELlSA微孔板，测定血清中的抗Fbal抗体，确定cutoff值并对方法的精密度和特异度进行考察。结果以重组Fbal抗原建立的ELlSA法精密度良好，批内变异系数（coefficientofvariation，CV）=1．81％，批间CV=14．85％；阻断率-88．8％，证实此法对抗白念珠菌Fbal抗体具有特异性。依据ROC曲线，选择吸光度（absorbance，A）值0．61作为cut—off值。IC患者阳性率为74．5％（82／110），念珠菌定植患者阳性率为12％（6／50），二者差异有统计学意义（x^2=7．8，P〈o．01）；健康体检者及细菌感染患者的抗体检出与IC患者相比，差异有统计学意义（p均〈0．001）。结论建立了检测抗白念珠菌Fbal抗体的ELISA法，在IC早期诊断中具有潜在应用价值。
Objective To establish an ELISA method with recombinant fructose-bisphosphate aldolase （Fbal） for detecting IgG antibody to Candida albicans Fbal in human sera,and evaluate its value in diagnosis of invasive candidiasis （IC）. Meth- ods Sera from 110 cases of patients with IC,50 cases with Candida spp. Colonization,147 cases with bacterial infections and 200 healthy subjects were collected. The ELISA method for detecting anti-Fbai antibody was established with recombinant Fbal as coating antigen,and the cut-off value,specificity and repeatability for the ELISA were determined. Results The in- tra-and inter-assay coefficients of variation for the developed ELISA were 1.81% and 14.85%, respectively. The blocking rate of sera with recombinant Fbal was 88.8%. According to ROC curve,the absorbance value of 0.61 was choseas the cut off value. In this condition,the sensitivity of the ELISA for diagnosis of IC was 74.5 % and the specificity was 92.8 %. The positive rate of antienolase antibody in the patients with IC （74.5%,82/110） was significantly higher than that of the pa- tients with Candida albicans colonization （12%, 6/50） （x2 = 7.8, P〈0.01）. Positive rate of healthy people and patients with bacterial infections compared with IC patients, the differences were statistically significant （P〈0. 01）. Conclusion Thc ELISA method for detecting the antibody to Candida albicans Fbal was established successfully, which may have potential value for early diagnosis of IC.
出处
《现代检验医学杂志》
CAS
2012年第5期22-24,28,共4页
Journal of Modern Laboratory Medicine
