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黄芪联合顺铂对喉癌Hep-2细胞的体外抑制作用 被引量:3

Inhibitory effect of cisplatin combined with Astragalus on human laryngeal carcinoma Hep-2 cells in-duced by
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摘要 目的研究黄芪与顺铂联合对体外培养的人喉癌Hep-2细胞的抑制作用。方法用MTT法检测黄芪、顺铂单独或联合作用对人喉癌Hep-2细胞的抑制率;用流式细胞技术及Western.blotting法检测黄芪、顺铂单独或联合作用对人喉癌Hep-2细胞凋亡的影响。结果作用24h后黄芪组、顺铂组、黄芪联合顺铂组对Hep-2细胞的抑制率分别为(53.83±17.12)%、(69.12±27.12)%、(84.55±27.84)%,与对照组(O%)比较差异有统计学意义(t=16.87,16.67,40.90,P〈0.01)。黄芪组、顺铂组、黄芪联合顺铂组Hep-2细胞的凋亡率[(38.2±13:6)%、(67.2±17.8)%、(86.4±25.1)%]与对照组[(17.1±1.3)%]比较差异有统计学意义(t=8.11,12.77,24.92,P〈0.05),且联合用药组的效果较任一单独用药组更为明显(t=11.33,9.37,P〈0.01)。结论黄芪通过促进细胞凋亡有效提高顺铂对Hep-2细胞抑制作用的敏感性,通过调节Bcl-2/Bax蛋白表达,能提高顺铂对喉癌的治疗效果。 Objective To study the anti-proliferation and apoptosis induction effect of cisplatin combined with Astragalus on human laryngeal cancer Hep-2 cells. Methods The proliferation inhibition of cisplatin and Astragalus alone or in combination on Laryngeal cancer Hep-2cells was measured by MTF as- say. Effects of cisplatin and Astragalus alone or in combination on apoptosis of Hep-2 cells were detected by flow cytometry (FCM). Western blot was used to analyze the expressions of Bcl-2 and Bax proteins. Re- sults The inhibition ratio of proliferation of Hep-2 cells was (53.83 ± 17. 12) % in the astragalus group, (69. 12 ± 27.12)% in the cisplatin group, and (84. 55 ± 27.84)% in the cisplatin combined with Astragalus group, and was significantly greater than the control group (0%) ( t = 16. 87, 16. 67,40. 90, P 〈 0. 01 ), respectively. The apoptotic ratio of Hep-2 cells was (38.2 ± 13.6)% in the astragalus group, (67.2±17.8 ) % in the cisplatin group, and ( 86.4 ± 25.1 ) %] in the cisplatin combined with Astragalus group, and was significantly greater than control group (17. 1±1.3 )% ( t = 8. 11,12. 77, 24. 92, P 〈 0.05), respectively. The effect in the combination group is better than the other group ( t = 11.33,9. 37, P 〈 0. 01 ). The expressions of Bcl-2 and Bax proteins were changed. Conclusions The tumor-killing effect of cisplatin on laryngeal cancer Hep-2 cells could be enhanced significantly by the combination appli- cation of astragalus by the way of regulating the expression of Bcl-2/Bax.
出处 《中国医师杂志》 CAS 2012年第10期1351-1353,共3页 Journal of Chinese Physician
基金 辽宁省自然科学基金项目(20082105),沈阳市科学技术计划项目(1091174-1-05)
关键词 喉肿瘤/药物疗法 喉肿瘤/中药疗法 顺铂/药理学 喉肿瘤/病理学 黄芪△/药理学 Laryngeal neoplasms/drug therapy Laryngeal neoplasms/ZHONG YAO LIAO FA Cisplatin/pharmacology Laryngeal neoplasms/pathology ASTRAGALUS MEMBRANACEUS A/pharmacology
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