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静电纺丝聚乳酸聚乙醇酸共聚物-丝素-胶原神经导管修复大鼠周围神经缺损 被引量:9

Repairing peripheral nerve defects in rats by electrostatic spinning poly (lactic-co-glycolic acid)-silk fibroin-collagen nerve conduits
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摘要 背景:周围神经缺损修复是临床上一大难题,由于自体神经移植有一定的局限性,人工神经修复材料是一种很有前途的选择。目的:探讨静电纺丝聚乳酸聚乙醇酸共聚物(PLGA)-丝素-胶原纳米神经导管修复大鼠坐骨神经缺损的可能性。方法:雌性SD大鼠36只,制备约10mm的坐骨神经缺损,分别采用倒转自体神经、静电纺丝PLGA-丝素-胶原神经导管、单纯PLGA神经导管桥接,术后12周进行大体观察、神经电生理测定、光镜观察、透射电镜观察和图像分析对比,了解神经再生的情况。结果与结论:静电纺丝法制备成的纳米神经导管管壁疏松多孔,能够模拟细胞外基质的结构。静电纺丝PLGA-丝素-胶原神经导管组在促进坐骨神经再生、提高再生神经髓鞘化、加速再生神经功能重建等方面均优于单纯PLGA导管组,比自体神经移植组略差。 BACKGROUND:The repair of peripheral nerve defects exhibits a clinical challenge.Because of the limitation of nerve autograft,it is a promising choice for artificial nerve grafts.OBJECTIVE:To investigate the possibility of repairing sciatic nerve defects by electrostatic spinning poly (lactic-co-glycolic acid) (PLGA)-silk fibroin-collagen nano nerve conduits.METHODS:Totally 36 female SD rats were underwent 10 mm sciatic nerve defects.The nerves of rats were grafted by reverse nerve autografts,electrostatic spinning PLGA-silk fibroin-collagen nerve conduits and PLGA nerve conduits alone.At week 12 postoperatively,nerve regeneration was studied by general observations,electrophysiological examination,microscopic observation,transmission electron microscopy observation and image analysis.RESULTS AND CONCLUSION:The results showed that the nanofibers-based nerve conduits prepared by electrostatic spinning had a well-distributed porous structure and could simulate the structure of native extracellular matrix.These findings suggest that the electrostatic spinning PLGA-silk fibroin-collagen nerve conduits group is superior to PLGA nerve conduits group in sciatic nerve regeneration,regenerative nerve myelinization and nerve foundation reconstruction,but slightly worse than the nerve autograft group.
出处 《中国组织工程研究》 CAS CSCD 2012年第38期7087-7091,共5页 Chinese Journal of Tissue Engineering Research
基金 国家自然科学基金资助项目(30772204) 四川省科技支撑计划项目(2100FZ0020)~~
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  • 1Scharpf J,Meirer R,Zielinski M. A novel technique for peripheral nerve repair[J].Laryngoscope,2003,(01):95-101.
  • 2Bini TB,Gao S,Wang S. Poly(l-lactide-co-glycolide) biodegradable microfibers and electrospun nanofibers for nerve tissue engineering:an in vitro study[J].Journal of Materials Science,2006,(19):6453-6459.
  • 3Nie HR,He AH,Jia B. A novel carrier of radionuclide based on surface modified poly(lactide-co-glycolide) nanofibrous membrane[J].Polymer,2010,(08):3344-3348.
  • 4Vol rath F,Knight DP. Liquid crystal ine spinning of spider silk[J].Nature,2001,(6828):541-548.
  • 5Stang F,Fansa H,Wolf G. Col agen nerve conduits--assessment of biocompatibility and axonal regeneration[J].Biomedical Materials & Engineering,2005,(1-2):3-12.
  • 6Al uin O,Wittmann C,Marqueste T. Functional recovery after peripheral nerve injury and implantation of a col agen guide[J].Biomaterials,2009,(03):363-373.
  • 7Min BM,Lee G,Kim SH. Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro[J].Biomaterials,2004,(7-8):1289-1297.
  • 8Rustemeyer J,Dicke U. Prefabricated nerve conduits advance histomorphological and functional outcomes in nerve regeneration of the sciatic nerve of the rat[J].Int J Oral Maxil ofac Surg,2010,(09):889-896.
  • 9江长青,胡军,向剑平,朱家恺,刘小林.猕猴组织工程化周围神经移植物的实验研究[J].中华手外科杂志,2005,21(6):372-374. 被引量:5
  • 10于海龙,彭江,孙华燕,徐风华,张莉,赵斌,眭翔,许文静,卢世璧.化学去细胞同种异体神经复合缓释神经生长因子修复周围神经缺损[J].中国修复重建外科杂志,2008,22(11):1373-1377. 被引量:33

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