摘要
目的探讨碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)对拟血管性痴呆(Vascular Dementia,VD)小鼠学习记忆障碍的保护作用。方法昆明小鼠80只,随机分为四组:正常组、假手术组、模型组、bFGF干预组(bFGF组)。采用清醒小鼠反复缺血再灌注制备VD动物模型。应用Morris水迷宫观察bFGF对VD小鼠学习记忆的改善作用;生化分析方法检测脑组织中SOD、MDA的含量。结果与正常组比较,模型组小鼠水迷宫试验的结果均有显著性差异(P<0.01),与模型组比较,bFGF组水迷宫试验的结果有显著性差异(P<0.01)。bFGF组脑组织SOD的活性增强,MDA的含量降低(P<0.01)。结论 bFGF具有改善血管性痴呆模型小鼠学习记忆障碍的作用,其机制可能与改善自由基代谢和脂质过氧化反应有关。
Objective To investigate the protective effect of basic fibroblast growth factor on learning-memory disorder in mice with vascular dementia and its mechanism.Methods Eighty healthy Kunming mice were divided randomly into 4 groups:control group,sham-operated group,model group and bFGF intervention group.Using repeated cerebral ischemia-reperfusion in conscious mice to establish the VD model.Infulence of bFGF on behavior were studied by Morris water maze.The change of levels of SOD(superoxide dismutase)and MDA(malondialdehyde)was analyzed with biochemistrical analysis method.Results In compared with the mice in model group,the protective effect of basic fibroblast growth factor on learning-memory disorder was improved significantly,the activity of SOD was remarkably inceased(P<0.01),MDA content was markedly reduced(P<0.01).Conclusion Basic fibroblast growth factor can improve learning-memory disorder in mice with vascular dementia.Its mechanism may be related to improve the metabolism of free radicals and lipid peroxidation.
出处
《中国老年保健医学》
2012年第5期31-32,共2页
Chinese Journal of Geriatric Care
