摘要
目的应用Meta分析的方法评价APC基因和P16基因甲基化与肝细胞癌(HCC)的关系。方法检索1978～2011年在Medline、Pubmed等数据库及1994～2011年在中国知网和万方数据库收录的所有有关P16、APC基因甲基化与HCC病例对照研究文献,按纳入与排除标准筛选文献,并用RevMan 5.0软件进行统计分析。结果纳入国内外标准的16篇数据合并结果显示,APC基因甲基化病例组488例,对照组HCC患者肿瘤组织434例,其中HCC癌旁组织390例,非肿瘤肝病患者组织21例,健康人组织23例;HCC组与癌旁组织组合并的有效率的比值比(OR)=5.91,95%CI为4.20%～8.31%;HCC肿瘤组与正常组织组OR=35.19,95%CI:6.00%～206.33%。P16甲基化病例组HCC患者肿瘤组织400例,对照组共437例,其中HCC癌旁组织398例,非肿瘤肝病患者组织21例,健康人组织18例。HCC组与癌旁组织组的OR=5.69,95%CI:3.96%～8.19%,HCC肿瘤组与正常组织组OR=12.18,95%CI:2.56%～58.03%。结论 APC、P16基因的高甲基化导致了该基因的失活,与HCC的发生和发展有密切关系。
Objective To evaluate the relationship of APC and P16 methylation with hepatocellular carcinoma（HCC） by meta analysis.Methods Articles related to the APC,P16 methylation in HCC published from 1978 to December 2011 in the databanks including MEDLINE and PUNMED and published from 1994 to December 2011 in databanks including CNKI,Wanfang database were collected,and data was analyzed by RevMan 5.0 software.Results Sixteen studies met our inclusion criteria,488HCCcases and 434 controls about APC methylation were brought into the study.Statistically significant odds ratios（ORs） of APC hypermethylation were obtained from tumor tissues and non-tumorous liver tissues of HCC patients（OR 5.91,95%CI：4.20%-8.31%）,tumor tissues of HCC patients and liver tissues of healthy person（OR 35.19,95%CI：6.00%-206.33%）.400 HCC cases and 437controls about P16 methylation were brought into the study.Statistically significant odds ratios（ORs） of P16 hypermethylation were obtained from tumor tissues and non-tumorous liver tissues of HCC patients（OR 5.69,95%CI：3.96%-8.19%）,tumor tissues of HCC patients and healthy liver tissues of healthy person（OR 12.18,95%CI：2.56%-58.03%）.Conclusion Hypermethylation of APC and P16 caused gene inactivation,and may contribute to the development of HCC.
出处
《检验医学与临床》
CAS
2012年第21期2698-2699,2702,共3页
Laboratory Medicine and Clinic
