摘要
[目的]对红掌组培快繁技术体系进行优化。[方法]以红掌红粉佳人品种为材料,通过设置不同的基本培养基和激素配比,研究红掌的不定芽诱导、增殖和生根等情况,以筛选出最佳的培养配方和方法。[结果]最佳愈伤组织诱导和不定芽分化培养基为MS+6-BA1.0 mg/L+2,4-D 0.1 mg/L+NAA 0.1 mg/L,其愈伤组织诱导率为73.3%,不定芽分化率为90.9%;在增殖培养基MS+6-BA 1.0 mg/L+NAA 0.2 mg/L上诱导出的芽数量与大小最适宜;生根培养以1/2MS+NAA 0.2 mg/L+活性炭1.0 g/L培养基表现最好;移栽基质以珍珠岩+草炭土(1∶1)混合栽培基质成活率最高,达95.6%。[结论]为红掌种苗的工厂化生产提供了参考。
[Objective] To optimize micropropagation of Anthurium andraeanurn.[Method] The effects of different basic medium and the combination of growth regulators on the adventitious bud inducement,proliferation and rootage were studied to find out the optimum formulation of Anthurium andraeanurn.[Result] The results showed that the best medium of callus inducement and adventitious bud differentiation was MS＋6-BA 1.0 mg/L＋2,4-D 0.1 mg/L＋NAA 0.1 mg/L.The rate of callus inducement and adventitious bud differentiation was 73.3% and 90.9% respectively.The suitable number and size were found on the proliferation medium MS＋6-BA 1.0 mg/L＋NAA 0.2 mg/L.The optimum rooting medium was 1/2MS ＋NAA 0.2 mg/L supplemented with active carbon 1.0 g/L.The adventitious shoots grew best with the survival rate 95.6% on the transplant medium which the ratio of perlite and peat soil is 1 to 1.[Conclusion] The study will provide reference for large-scale production of Anthurium andraeanurn.
出处
《安徽农业科学》
CAS
2012年第33期16052-16053,16080,共3页
Journal of Anhui Agricultural Sciences
关键词
红掌
组织培养
优化
最佳配方
Anthurium andraeanurn
Tissue culture
Optimization
Optimum formulation
