摘要
背景:骨形态发生蛋白7可以促进椎间盘组织合成细胞外基质。目的:观察椎间盘退变兔模型骨形态发生蛋白7的表达。方法:新西兰大白兔采用髓核抽吸法建立椎间盘退变动物模型,对照组暴露相应椎间隙后不进行任何干预。建模成功后,连续核磁共振观察椎间盘变化,12周后生化分析椎间盘中蛋白多糖含量,同时免疫组织化学检测退变的椎间盘组织中Ⅱ型胶原和骨形态发生蛋白7的表达。结果与结论:建模后2周,核磁共振发现模型兔椎间盘开始退变,出现椎间隙狭窄,T2像上椎间盘信号强度减弱。建模后12周,模型兔椎间隙高度、T2信号强度、蛋白多糖含量、Ⅱ型胶原和骨形态发生蛋白7的表达均低于对照组(P<0.05)。结果证实,实验采用髓核抽吸法成功建立了退变椎间盘兔模型,其退变的椎间盘组织中骨形态发生蛋白7的表达下降。
BACKGROUND：Bone morphogenetic protein-7 can promote extracellular matrix synthesis in the intervertebral disc tissue. OBJECTIVE：To observe the expression of bone morphogenetic protein-7 in rabbits with intervertebral disc degeneration. METHODS：Models of intervertebral disc degeneration were established in New Zealand white rabbits using nucleus aspiration. The lumbar intervertebral disc of L3/4, L4/5, L5/6 were exposed in control group without treatment. The changes in the intervertebral disc were observed by MRI after modeling. The contents of bone morphogenetic protein-7, proteoglycan and type Ⅱ collagen were measured in 12 weeks later. RESULTS AND CONCLUSION：Intervertebral disc degeneration occurred at 2 weeks after modeling detected by using MRI. Compared with the control group, the disc height, T2 value, contents of bone morphogenetic protein-7, proteoglycan and type Ⅱ collagen in the model group were significantly decreased at 12 weeks after modeling. It indicated that the expression of bone morphogenetic protein-7 was declined in the degenerative intervertebral disc of rabbits induced by nucleus aspiration method.
出处
《中国组织工程研究》
CAS
CSCD
2012年第37期6871-6875,共5页
Chinese Journal of Tissue Engineering Research
