摘要
目的:探讨泌乳素相关蛋白(PIP)在前列腺癌不同细胞系中的表达情况,以及雄激素对其表达的影响。方法:应用不同浓度(0、0.1、1、10、100nmol/L)双氢睾酮(Dihydrotestosterone,DHT)或睾酮(Testosterone,T)及不同时间(6、24和48h)对前列腺癌细胞系(LNCaP、PC-3、DU145)及人乳腺癌细胞系T47D进行处理,通过RT-PCR及实时荧光定量PCR法检测PIP及雄激素受体(AR)mRNA表达情况。通过Westernblot法检测各细胞系(LNCaP、PC-3、DU145、T47D)中PIP蛋白表达情况。结果:雄激素依赖性前列腺癌细胞系LNCaP中有PIP mRNA及蛋白表达,PI P mRNA的表达随DHT或T浓度增加而增加,且于10nmol/L浓度DHT或T时达到最大峰值。而AR mRNA的表达差异无统计学意义。雄激素非依赖性前列腺癌细胞系PC-3与DU145中无PIP mRNA及蛋白表达,并且AR表达较少或无AR表达。结论:PIP在前列腺癌发生发展机制中,以及鉴别前列腺癌是否具有雄激素依赖性方面有一定作用。
Objective： To determine prolactin-inducible protein （PIP） mRNA expression and effects of androgen in human prostate cancer cell lines. Methods： Prostate cell hnes （LNCaP, PC-3, DU145） and breast tumor cell line （T47D） were treated by different concentrations （0, 0.1, 1, 10 and 100 nmol/L） and different times （6, 24 and 48 h） of dihydrotestosterone （DHT） or testosterone （T）. The PIP and androgen receptor （AR） mRNA expressions were detected by RT-PCR. The PIP protein expression was determined by Western blot in four cell lines. Results： By RT-PCR and Western blot, PIP mRNA and protein expression were found in androgen-dependent prostate cancer cell line LNCaP. PIP mRNA expression was increased along with concentrations of DHT or T in LNCaP. PIP mRNA level reached maximum peak in 10 nmol/L DHT or T. There was no significant difference in AR mRNA expression. There were no PIP mRNA and protein expressions in androgen-independent prostate cancer cell lines PC-3 and DU145. Conclusion： The role of PIP may occur in the process of development of prostate cancer, and in the differentialdiagnosis of prostate cancer for the androgen dependence.
出处
《天津医药》
CAS
北大核心
2012年第11期1110-1113,共4页
Tianjin Medical Journal
基金
河北省卫生厅重点课题计划(项目编号:20090543)
关键词
前列腺肿瘤
雄激素类
催乳素
前列腺特异抗原
睾酮
双氢睾酮
聚合酶链反应
prostatic neoplasms androgens prolactin prostate-specific antigen testosterone dihydrotestosteronepolymerase chain reaction
