摘要
以‘绿岭’核桃实生幼苗为材料,研究掐茎尖和秋水仙素对幼苗生长及倍性的影响。结果表明:处理C即未掐茎尖的核桃幼苗在第1～7天的高生长量为13.63cm,极显著高于处理C1(掐茎尖加蒸馏水处理)、C2～C6(掐茎尖加0.1%、0.2%、0.4%、0.6%、0.8%秋水仙素处理);各处理植株上胚轴上平均膨大的芽数以C1最高,为8.1个,极显著高于其他处理,表明掐茎尖和秋水仙素处理明显抑制核桃幼苗主茎生长,促进上胚轴上芽的萌发伸长;随着秋水仙素浓度的增加,植株上胚轴停长时间由处理第8～9天提前到第1天,植株顶端变黑萎蔫长度由切口发黑增大到2.48cm,上胚轴上平均萌发伸长的芽数由1.7个增加到3.0个;处理C2和C3出现二倍体细胞和四倍体细胞构成的混倍体叶片。
＇Lv Ling＇ walnut seedlings were used as the experimental materials to study the effects of top-pinching and colehicine on the growth and ploidy of walnut seedlings. The results showed that： the height growth of ＇Lv Ling＇ walnut seedlings was 13.63 cm on the first to sev enth day by treatment C（no top-pinching）, significantly higher than that of Cl（top-pinching and dipped distilled water ）, C2-C6（top-pinching and treated with 0. 1%, 0. 2%, 0. 4%, 0. 6%, 0. 8% colchicine） . The average quantity of germinate buds was 8.1 in treatment C1, which was sig nificantly higher than that of others. It indicated that treating with top-pinching and colchicine inhibited growing of walnut seedlings, but it promoted the buds germination. With the increas ing of colchicine concentration, the time of seedlings stoped growing preset on the first dayfrom the eighth day to nineth day, dead tip extended from zero point to 2.48 cm on the epicot yl, the average quantity of buds on epicoty increased from 1.7 to 3. O. MixopIo^d leaf which constitute with diploid and tetraploid were found in C2 and C3.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2012年第5期29-33,共5页
Journal of Hebei Agricultural University
基金
河北省科技支撑项目(11230115D)
关键词
核桃
掐茎尖
秋水仙素
生长
倍性
walnut
top-pinching colchicine growth ploidy
